CAJ | 학술논문

目的：探讨外源性磷酸肌酸预处理对大鼠子宫缺血再灌注损伤的保护作用及其机制。方法选择 SD雌性大鼠45只,随机分为假手术组、缺血再灌注组和干预组,每组15只。干预组于缺血前30 min 经腹腔注射磷酸肌酸注射液10 mg/ kg,测定子宫组织中超氧化物歧化酶(SOD)活性和丙二醛(MDA)水平,并观察子宫病理组织学改变。结果与假手术组比较,缺血再灌注组和干预组大鼠子宫组织 SOD 活性明显降低,MDA 含量明显增加(P <0．05)；与缺血再灌注组比较,干预组 SOD 活性升高,MDA 含量降低(P <0．05)。干预组病理组织学观察炎性反应较缺血再灌注组明显减轻。结论磷酸肌酸对大鼠子宫缺血再灌注损伤有保护作用,其机制可能与其增强 SOD 活性有关。
목적：탐토외원성린산기산예처리대대서자궁결혈재관주손상적보호작용급기궤제。방법선택 SD자성대서45지,수궤분위가수술조、결혈재관주조화간예조,매조15지。간예조우결혈전30 min 경복강주사린산기산주사액10 mg/ kg,측정자궁조직중초양화물기화매(SOD)활성화병이철(MDA)수평,병관찰자궁병리조직학개변。결과여가수술조비교,결혈재관주조화간예조대서자궁조직 SOD 활성명현강저,MDA 함량명현증가(P <0．05)；여결혈재관주조비교,간예조 SOD 활성승고,MDA 함량강저(P <0．05)。간예조병리조직학관찰염성반응교결혈재관주조명현감경。결론린산기산대대서자궁결혈재관주손상유보호작용,기궤제가능여기증강 SOD 활성유관。
Objective To study the protective effect and its mechanisms of exogenous creatine phosphate (PCr) preconditioning on uterine ischemia-reperfusion injury in rats. Methods A total of 45 SD female rats were randomly di-vided into sham operation group (n = 15), ischemia-reperfusion group (n = 15) and intervention group (n = 15). The intervention group was given 10 mg/ kg PCr injection via intraperitoneal injection 30 min before ischemia. The activity of superoxide dismutase (SOD) and malondialdehyde (MDA) level in uterine tissues were detected, and the pathological changes of uterus were also observed. Results Compared with those in the sham operation group, the SOD activities were significantly reduced, while MDA levels were significantly increased in ischemia-reperfusion and intervention groups (P < 0. 05); compared with those in ischemia-reperfusion group, the SOD activity was significantly increased, and MDA level was significantly reduced in the intervention group (P < 0. 05). Histopathological observation showed that inflamma-tory reaction in the intervention group was significantly alleviated compared with that in the ischemia-reperfusion group. Conclusion PCr may protect uterine ischemia-reperfusion injury to a certain degree in rats, and the mechanisms may be related with its activation to SOD activity.