解放军医药杂志
解放軍醫藥雜誌
해방군의약잡지
MEDICAL&PHARMACEUTICAL JOURNAL OF CHINESE PEOPLE'S LIBERATION ARMY
2015年
5期
13-16
,共4页
李冬秀%耿杨柳%吴小华%张敏%徐清华%于晓惠%许鹏宇
李鼕秀%耿楊柳%吳小華%張敏%徐清華%于曉惠%許鵬宇
리동수%경양류%오소화%장민%서청화%우효혜%허붕우
卵泡刺激素%血管内皮生长因子类%卵巢%低温保存%移植,自体%大鼠,Wistar
卵泡刺激素%血管內皮生長因子類%卵巢%低溫保存%移植,自體%大鼠,Wistar
란포자격소%혈관내피생장인자류%란소%저온보존%이식,자체%대서,Wistar
Follicle stimulating hormone%Vascular endothelial growth factors%Ovary%Cryopreservation%Trans-plantation,autologous%Rats,Wistar
目的：探讨促卵泡激素(FSH)和血管内皮生长因子(VEGF)在大鼠卵巢组织冷冻自体异位移植后对卵泡内分泌功能的影响。方法 Wistar 大鼠30只随机分为 A、B、C 组,每组10只,A 组为新鲜卵巢组织移植,B 组和 C 组卵巢组织均直接覆盖玻璃化冷冻保存2周复苏后移植,但 C 组在移植前2 d 肌内注射 FSH 和 VEGF,连续用药5 d。移植6周后计算卵泡密度；检测大鼠血清中雌二醇(E2)水平；卵巢组织增殖细胞核抗原(PCNA)的表达情况,并测定子宫重量。结果 A、B、C 组移植后卵巢组织存活率比较差异无统计学意义(P >0．05)。 A、C 组卵泡密度、血清 E2水平、PCNA 阳性率和子宫重量均高于 B 组(P <0．05),A 组和 C 组比较差异无统计学意义(P >0．05)。结论大鼠卵巢组织冷冻复苏自体异位移植前注射 FSH 和 VEGF 可以有效提高卵泡存活率,有利于恢复卵巢内分泌功能。
目的：探討促卵泡激素(FSH)和血管內皮生長因子(VEGF)在大鼠卵巢組織冷凍自體異位移植後對卵泡內分泌功能的影響。方法 Wistar 大鼠30隻隨機分為 A、B、C 組,每組10隻,A 組為新鮮卵巢組織移植,B 組和 C 組卵巢組織均直接覆蓋玻璃化冷凍保存2週複囌後移植,但 C 組在移植前2 d 肌內註射 FSH 和 VEGF,連續用藥5 d。移植6週後計算卵泡密度；檢測大鼠血清中雌二醇(E2)水平；卵巢組織增殖細胞覈抗原(PCNA)的錶達情況,併測定子宮重量。結果 A、B、C 組移植後卵巢組織存活率比較差異無統計學意義(P >0．05)。 A、C 組卵泡密度、血清 E2水平、PCNA 暘性率和子宮重量均高于 B 組(P <0．05),A 組和 C 組比較差異無統計學意義(P >0．05)。結論大鼠卵巢組織冷凍複囌自體異位移植前註射 FSH 和 VEGF 可以有效提高卵泡存活率,有利于恢複卵巢內分泌功能。
목적：탐토촉란포격소(FSH)화혈관내피생장인자(VEGF)재대서란소조직냉동자체이위이식후대란포내분비공능적영향。방법 Wistar 대서30지수궤분위 A、B、C 조,매조10지,A 조위신선란소조직이식,B 조화 C 조란소조직균직접복개파리화냉동보존2주복소후이식,단 C 조재이식전2 d 기내주사 FSH 화 VEGF,련속용약5 d。이식6주후계산란포밀도；검측대서혈청중자이순(E2)수평；란소조직증식세포핵항원(PCNA)적표체정황,병측정자궁중량。결과 A、B、C 조이식후란소조직존활솔비교차이무통계학의의(P >0．05)。 A、C 조란포밀도、혈청 E2수평、PCNA 양성솔화자궁중량균고우 B 조(P <0．05),A 조화 C 조비교차이무통계학의의(P >0．05)。결론대서란소조직냉동복소자체이위이식전주사 FSH 화 VEGF 가이유효제고란포존활솔,유리우회복란소내분비공능。
Objective To investigate the effects of follicle stimulating hormone (FSH) and vascular endothelial growth factor (VEGF) on follicular endocrinic function of ovarian tissues after vitrification and autologous heterotopic transplantation. Methods A total of 30 female Wistar rats were randomly divided into group A, B and C (n = 10 for each group). Group A was transplanted with fresh tissues; group B and C were transplanted with resurgent tissues after cryopreserved for 2 weeks by direct cover vitrification (DCV) method, but group C received FSH and VEGF with intra-muscular injection 2 d before the transplantation for consecutive 5 d. After heterotopic auto transplantation for 6 weeks, values of follicular density were calculated; serum estradiol (E2 ) levels were detected; expressions of proliferating cell nuclear antigen (PCNA) were detected, and the values of uterine weight were measured. Results There were no statis-tically significance differences in survival rates of ovarian tissues among the 3 groups (P > 0. 05). The values of follicular density, serum E2 level, positive rate of PCNA and uterine weight in group A and C were higher than those in group B (P < 0. 05), but there were no statistically significant differences in the values between group A and C (P > 0. 05). Conclusion VEGF and FSH of intramuscular injection before the transplantation for ovarian tissues after vitrification and resuscitation can effectively increase the survival rate of ovarian tissues and improve the recovery of follicular endocrinic function.
