CAJ | 학술논문

为明确水稻黑条矮缩病毒(Rice black streaked dwarf virus ,RBSDV)侵染对水稻脱落酸(abscisic acid,ABA)含量的影响,通过灰飞虱在水稻日本晴和淮稻5号两个品种上人工接种 RBSDV,待接种的水稻表现明显矮缩症状时,采用 ELISA方法测定 ABA 含量.结果显示,在接种 RBSDV 的日本晴和淮稻5号中,ABA 含量均明显增加.接种病毒的日本晴植株中ABA 含量为111．04 ng/g,而对照中仅为60．86 ng/g；淮稻5号对照植物 ABA 含量为70．61 ng/g,而病株中 ABA 的含量为102．60 ng/g.为进一步明确病毒侵染如何调控 ABA 的含量,采用荧光定量 PCR 方法分析了日本晴接种 RBSDV 8 d,12 d,16 d 和60 d 时 ABA 合成关键基因(OsZEP 、OsNCED1、OsNCED2、OsNCED3、OsNCED4和 OsNCED5)及分解关键基因(OsABA8ox1、OsABA8ox2和 OsABA8ox3)mRNA 相对表达量的变化.结果显示,病毒侵染8 d,ABA 合成和分解代谢基因的表达量均高于对照,其中 OsNCED4和 OsNCED5的表达量随病毒侵染时间的延长而增加,至60 d 时 OsNCED3、OsNCED4和 OsNCED5的表达量为对照的3．97、7．66和2．99倍,而 OsZEP ,OsABA8ox1和 OsABA8ox2的表达量则随侵染时间的延长而降低.RBSDV 侵染后可能既影响了 ABA 合成也影响了其分解,两者共同作用导致了 ABA 含量增加.
위명학수도흑조왜축병독(Rice black streaked dwarf virus ,RBSDV)침염대수도탈락산(abscisic acid,ABA)함량적영향,통과회비슬재수도일본청화회도5호량개품충상인공접충 RBSDV,대접충적수도표현명현왜축증상시,채용 ELISA방법측정 ABA 함량.결과현시,재접충 RBSDV 적일본청화회도5호중,ABA 함량균명현증가.접충병독적일본청식주중ABA 함량위111．04 ng/g,이대조중부위60．86 ng/g；회도5호대조식물 ABA 함량위70．61 ng/g,이병주중 ABA 적함량위102．60 ng/g.위진일보명학병독침염여하조공 ABA 적함량,채용형광정량 PCR 방법분석료일본청접충 RBSDV 8 d,12 d,16 d 화60 d 시 ABA 합성관건기인(OsZEP 、OsNCED1、OsNCED2、OsNCED3、OsNCED4화 OsNCED5)급분해관건기인(OsABA8ox1、OsABA8ox2화 OsABA8ox3)mRNA 상대표체량적변화.결과현시,병독침염8 d,ABA 합성화분해대사기인적표체량균고우대조,기중 OsNCED4화 OsNCED5적표체량수병독침염시간적연장이증가,지60 d 시 OsNCED3、OsNCED4화 OsNCED5적표체량위대조적3．97、7．66화2．99배,이 OsZEP ,OsABA8ox1화 OsABA8ox2적표체량칙수침염시간적연장이강저.RBSDV 침염후가능기영향료 ABA 합성야영향료기분해,량자공동작용도치료 ABA 함량증가.
To investigate the effect of virus infection on abscisic acid (ABA)content in rice,two rice varieties, Nipponbare and Huaidao 5 ,were inoculated with rice black streaked dwarf virus (RBSDV)artificially via small brown planthoppers,and the ABA content was measured by ELISA method when the plants showed the obvious viral disease symptoms.The results showed that the ABA content was increased in virus infected rice.The ABA content in virus infected Nipponbare was 1 1 1 .04 ng/g compared to 60.86 ng/g in the control.In Huaidao 5 ,it was 102.60 ng/g in the virus infected plants and 70.6 1 ng/g in the control.To reveal how the virus regulates the ABA content,the relative expression levels of genes involved in ABA biosynthesis (OsZEP ,OsNCED1 ,OsNCED2 ,OsNCED3 ,OsNCED4 and OsNCED5 )and catabolism (OsABA8ox1 ,OsABA8ox2 and OsABA8ox3 )were analyzed in Nipponbare at 8 d,12 d, 1 6 d and 60 d after virus infection.The qRT-PCR results showed that the expression levels of the genes involved in ABA biosynthesis and catabolism in the plants than at 8 days after virus infection were higher than those in the control. The expression levels of OsNCED4 and OsNCED5 increased continuously with infection duration.At 60 d,the expression levels of OsNCED3 ,OsNCED4 and OsNCED5 in RBSDV infected plants were 3.97-,7.66-and 2.99-fold higher than those in the control,and the expression levels of OsZEP ,OsABA8ox1 and OsABA8ox2 were decreased with time extension.The data suggested that RBSDV infection affected the ABA biosynthesis and catabolism metabolism pathway,resulting in the increase of ABA content.