CAJ | 학술논문

以稻曲病菌致病力丧失的突变菌株 B1464为材料，对其生物学形态和分子遗传变异进行分析.B1464田间接种表现为不致病，与野生型菌株相比，在营养贫瘠的 MM 培养基上生长速率没有显著差异，而在 PSA 和 TB3培养基中生长速率较慢，并且在 PS 液体培养基中产孢能力下降，同时突变菌株的分生孢子与野生型相比形态和大小均发生变化.Southern 杂交结果显示 T-DNA 在突变菌株 B1464中以单拷贝形式插入，利用 TAIL-PCR 技术扩增得到的紧邻 T-DNA 两侧的侧翼序列在野生型中不相邻，与野生型菌株基因组比对发现突变菌株插入位点处丢失约20 kb 的 DNA 片段.RT-PCR 结果表明突变菌株中 T-DNA 插入位点两侧基因表达量均下调.推断突变菌株 B1464的致病能力丧失可能是由于 T-DNA 的插入导致了染色体重排及破坏了某些基因的正常表达.
이도곡병균치병력상실적돌변균주 B1464위재료，대기생물학형태화분자유전변이진행분석.B1464전간접충표현위불치병，여야생형균주상비，재영양빈척적 MM 배양기상생장속솔몰유현저차이，이재 PSA 화 TB3배양기중생장속솔교만，병차재 PS 액체배양기중산포능력하강，동시돌변균주적분생포자여야생형상비형태화대소균발생변화.Southern 잡교결과현시 T-DNA 재돌변균주 B1464중이단고패형식삽입，이용 TAIL-PCR 기술확증득도적긴린 T-DNA 량측적측익서렬재야생형중불상린，여야생형균주기인조비대발현돌변균주삽입위점처주실약20 kb 적 DNA 편단.RT-PCR 결과표명돌변균주중 T-DNA 삽입위점량측기인표체량균하조.추단돌변균주 B1464적치병능력상실가능시유우 T-DNA 적삽입도치료염색체중배급파배료모사기인적정상표체.
With an avirulent Ustilaginoidea virens strain B1464 as material,we analyzed the changes in biological morphology and pathogenic mechanism.Compared to the wild-type U .virens strain P1 ,the mutant strain B1464 showed no pathogenicity in the field.The rate of growth on MM medium was not different to P1 and B1464.On PSA and TB3 medium the rates of growth were nutritionally endowed.B1464 grew slower and produced few conidiophores in PS medium, and its conidiophores morphology and spore size became smaller.Genomic Southern bolt analysis confirmed that B1464 was single T-DNA insertional events.The flanking sequences of T-DNA obtained by TAIL-PCR were non-adjacent in the wild type.It was founds that the mutant strain lost about 20 kb sequences in the insertion site comparing with the wild type strain.The expression of the flanking genes of the T-DNA was down-regulated,detecting by semi-quantitative RT-PCR.The reason of the chromosome rearrangement and abnormal expression of some genes is the insertion of T-DNA,probably,leading to the mutant strain B1464 lost its pathogenicity.