CAJ | 학술논문

通过对已克隆的抗稻瘟病基因 Pi2、Pi9以及 Piz-t 进行序列比对，寻找各自特异的核苷酸差异，成功开发了基于PCR 技术以及电泳检测技术的 Pi2/Pi9/Piz-t 以及 Piz-t 的基因特异性分子标记，能有效地将 Pi2/Pi9/Piz-t 与该位点上的其他抗性等位基因及感病等位基因区分开，这为分子标记辅助育种及抗病基因聚合提供有效的分子标记。用 Pi2/Pi9/Piz-t 基因特异性分子标记对来自全国各稻区的共101份水稻品种和育种亲本进行分子检测，结果发现，除2个品种检测到Piz-t 带型之外，大部分水稻品种不携带这3个抗性基因，这为有目的地开展品种的抗性改良提供了重要的参考信息。
통과대이극륭적항도온병기인 Pi2、Pi9이급 Piz-t 진행서렬비대，심조각자특이적핵감산차이，성공개발료기우PCR 기술이급전영검측기술적 Pi2/Pi9/Piz-t 이급 Piz-t 적기인특이성분자표기，능유효지장 Pi2/Pi9/Piz-t 여해위점상적기타항성등위기인급감병등위기인구분개，저위분자표기보조육충급항병기인취합제공유효적분자표기。용 Pi2/Pi9/Piz-t 기인특이성분자표기대래자전국각도구적공101빈수도품충화육충친본진행분자검측，결과발현，제2개품충검측도Piz-t 대형지외，대부분수도품충불휴대저3개항성기인，저위유목적지개전품충적항성개량제공료중요적삼고신식。
Specific molecular markers for detecting the alleles of Pi2/Pi9/Piz-t ,designated as Pi2SNP,Pi9SNP and Pizt-PA,respectively,have been successfully developed through sequence comparison method.These three specific markers can distinctly distinguish target genes from others mapped on Pi2/9 locus,which facilitate the molecular marker-assistant selection and the development of pyramiding cultivars combined with other allele R genes. Additionally,101 main cultivars and breeding parents collected from different rice-planting areas have been diagnosed with these three specific markers,and the results showed that only 2 out of 101 cultivars carried Piz-t ,but neither Pi2 nor Pi9 band had been detected.So far,it indicated that most of the main cultivars and breeding parents in China do not carry Pi2/Pi9/Piz-t .These results had provided important information for the application of Pi2/Pi9/Piz-t in the blast resistance breeding programs.