药学实践杂志
藥學實踐雜誌
약학실천잡지
THE JOURNAL OF PHARMACEUTICAL PRACTICE
2015年
3期
242-245,274
,共5页
方凤凯%汤华%刘宝姝%庄春林%孙鹏%张文
方鳳凱%湯華%劉寶姝%莊春林%孫鵬%張文
방봉개%탕화%류보주%장춘림%손붕%장문
群海绵%二萜生物碱%aurantiamide acetate%结构鉴定%肿瘤生长抑制活性
群海綿%二萜生物堿%aurantiamide acetate%結構鑒定%腫瘤生長抑製活性
군해면%이첩생물감%aurantiamide acetate%결구감정%종류생장억제활성
A gelas mauritiana%diterpene alkaloid%aurantiamide acetate%structure identification%tumor cell grow th in-hibitory activity
目的:对采自南中国海海域的群海绵(A gelas mauritiana)的化学成分进行研究。方法采用硅胶色谱柱、Sephadex L H-20凝胶色谱柱、高效液相色谱等色谱方法,对 A gelas mauritiana正丁醇萃取物进行分离纯化;应用现代波谱技术对化合物进行化学结构鉴定;用M T T法对化合物进行体外人肺癌细胞株A549细胞生长抑制活性测试。结果共分离得到8个化合物,分别鉴定为:agelasine A (1)、agelasine B (2)、epi-agelasine C (3)、(-)agelasine D (4)、agelasine E (5)、agelasine F (6)、(-)ageloxime D (7)、aurantiamide acetate (8)。体外活性筛选中,这些化合物对A549显示出不同程度的生长抑制活性,化合物1~3的活性与阳性对照阿霉素相近。结论化合物1、2、4、5、6、8为首次从该种海绵中分离得到。首次选用A549对化合物1~7的活性进行评价,化合物2、3的显著生长抑制活性为进一步深入研究提供了依据。
目的:對採自南中國海海域的群海綿(A gelas mauritiana)的化學成分進行研究。方法採用硅膠色譜柱、Sephadex L H-20凝膠色譜柱、高效液相色譜等色譜方法,對 A gelas mauritiana正丁醇萃取物進行分離純化;應用現代波譜技術對化閤物進行化學結構鑒定;用M T T法對化閤物進行體外人肺癌細胞株A549細胞生長抑製活性測試。結果共分離得到8箇化閤物,分彆鑒定為:agelasine A (1)、agelasine B (2)、epi-agelasine C (3)、(-)agelasine D (4)、agelasine E (5)、agelasine F (6)、(-)ageloxime D (7)、aurantiamide acetate (8)。體外活性篩選中,這些化閤物對A549顯示齣不同程度的生長抑製活性,化閤物1~3的活性與暘性對照阿黴素相近。結論化閤物1、2、4、5、6、8為首次從該種海綿中分離得到。首次選用A549對化閤物1~7的活性進行評價,化閤物2、3的顯著生長抑製活性為進一步深入研究提供瞭依據。
목적:대채자남중국해해역적군해면(A gelas mauritiana)적화학성분진행연구。방법채용규효색보주、Sephadex L H-20응효색보주、고효액상색보등색보방법,대 A gelas mauritiana정정순췌취물진행분리순화;응용현대파보기술대화합물진행화학결구감정;용M T T법대화합물진행체외인폐암세포주A549세포생장억제활성측시。결과공분리득도8개화합물,분별감정위:agelasine A (1)、agelasine B (2)、epi-agelasine C (3)、(-)agelasine D (4)、agelasine E (5)、agelasine F (6)、(-)ageloxime D (7)、aurantiamide acetate (8)。체외활성사선중,저사화합물대A549현시출불동정도적생장억제활성,화합물1~3적활성여양성대조아매소상근。결론화합물1、2、4、5、6、8위수차종해충해면중분리득도。수차선용A549대화합물1~7적활성진행평개,화합물2、3적현저생장억제활성위진일보심입연구제공료의거。
Objective To investigate the chemical constituents of marine sponge A gelas mauritiana collected from the South China Sea .Methods The n-butanol extract of marine sponge A gelas mauritiana was separated and purified by repeated column chromatography on silica gel ,Sephadex LH-20 ,and reversed-phase high-performance liquid chromatography (RP-HPLC) .The chemical structures of those obtained compounds were determined on the basis of spectroscopic analysis and com-parison with reported data .The tumor cell growth inhibitory activity of these compounds towards human lung carcinoma cells A549 was tested .Results Eight compounds were isolated ,including agelasine A (1) ,agelasine B (2) ,epi-agelasine C (3) , (-)agelasine D (4) ,agelasine E (5) ,agelasine F (6) ,(-)ageloxime D (7) and aurantiamide acetate (8) .These compounds dis-played different level of tumor cell growth inhibitory activity towards cell A 549 in vitro .Compounds 1-3 showed significant ac-tivity towards cell A549 ,being similar to that of the positive control of adriamycin .Conclusion Compounds 1 ,2 ,4 ,5 ,6 ,8 were isolated for the first time from the sponge A gelas mauritiana .Cell A549 was selected for the first time for the activity evalua-tion of compounds 1~7 .Significant inhibition activity of compounds 2、3 may hold as a basis for further research .