CAJ | 학술논문

目的：研究大孔树脂分离纯化栀子豉汤有效成分的工艺条件及优化。方法采用紫外分光光度法测定总环烯醚萜苷和总大豆异黄酮的量，以总环烯醚萜苷和总大豆异黄酮的含量作为指标，采用单因素实验考察上样液浓度、上样量、洗脱剂浓度及用量等对分离纯化工艺的影响。结果最佳工艺条件：上样液最佳吸附浓度为0．1 g／ml（该浓度以每1 ml中所含生药材质量计），上样量与树脂体积比是2∶1，吸附时间2h，依次用1BV（柱体积）水，6BV20％乙醇洗脱，6BV60％乙醇洗脱，收集洗脱液。结论该纯化工艺合理、稳定，可推广于工业生产。
목적：연구대공수지분리순화치자시탕유효성분적공예조건급우화。방법채용자외분광광도법측정총배희미첩감화총대두이황동적량，이총배희미첩감화총대두이황동적함량작위지표，채용단인소실험고찰상양액농도、상양량、세탈제농도급용량등대분리순화공예적영향。결과최가공예조건：상양액최가흡부농도위0．1 g／ml（해농도이매1 ml중소함생약재질량계），상양량여수지체적비시2∶1，흡부시간2h，의차용1BV（주체적）수，6BV20％을순세탈，6BV60％을순세탈，수집세탈액。결론해순화공예합리、은정，가추엄우공업생산。
Objective To optimize separation and purification technology of Zhizichi decoction by macroporous resina . Methods The content of total iridoid glycosides and total isoflavones were determined by ultraviolet spectrophotometry .Tak-ing the content of total iridoid glycosides and total isoflavones as indexes ,the effect of the concentration of sample solution , medicinal herbs on the amount of sample ,concentration and volume of eluent was investigated by single factor test .Results The optimum separation and purification technology was as following :the concentration of sample solution was 0 .1 g/ml ,the volume ratio of resin to material drug was 2∶1 ,the adsorption time was 2 h ,and the sample was firstly eluted with 1 BV wa-ter ,then 6 BV 20% ethanol and 60% ethanol ,and the eluent was collected .Conclusion This optimized separation and purifi-cation technology was reasonable and stable ,and it could be extended to large-scale production applications .