CAJ | 학술논문

目的：确定黎药裸花紫珠的止血活性部位，为其止血活性成分的研究提供依据。方法裸花紫珠经醇提获得醇提物（EtOH-extract）、醇提物经大孔吸附树脂处理分别获得水洗脱物（HP-H2 O-elution）和乙醇洗脱物（HP-EtOH-elution），将EtOH-extract、HP-EtOH-elution和HP-H2 O-elution分别配制成高、中、低剂量的供试药物，云南白药为阳性对照。每日给小鼠灌胃1次，在连续给药7d后，记录小鼠体质量的变化，采用断尾法和玻片法测定小鼠的出血时间和凝血时间，并以此为筛选指标对各部位止血效果进行评价。结果与模型对照组相比，HP-EtOH-elution对缩短小鼠出血时间的作用效果显著，并且其低剂量组和中剂量组的效果均优于阳性对照组。EtOH-extract、HP-EtOH-elution和HP-H2O-elution在缩短小鼠的凝血时间和增加小鼠的体质量方面，效果均不显著。结论裸花紫珠的止血活性成分主要集中在醇提物经大孔树脂处理后的乙醇洗脱部位，并且可能是通过影响内源性凝血途径发挥止血作用。
목적：학정려약라화자주적지혈활성부위，위기지혈활성성분적연구제공의거。방법라화자주경순제획득순제물（EtOH-extract）、순제물경대공흡부수지처리분별획득수세탈물（HP-H2 O-elution）화을순세탈물（HP-EtOH-elution），장EtOH-extract、HP-EtOH-elution화HP-H2 O-elution분별배제성고、중、저제량적공시약물，운남백약위양성대조。매일급소서관위1차，재련속급약7d후，기록소서체질량적변화，채용단미법화파편법측정소서적출혈시간화응혈시간，병이차위사선지표대각부위지혈효과진행평개。결과여모형대조조상비，HP-EtOH-elution대축단소서출혈시간적작용효과현저，병차기저제량조화중제량조적효과균우우양성대조조。EtOH-extract、HP-EtOH-elution화HP-H2O-elution재축단소서적응혈시간화증가소서적체질량방면，효과균불현저。결론라화자주적지혈활성성분주요집중재순제물경대공수지처리후적을순세탈부위，병차가능시통과영향내원성응혈도경발휘지혈작용。
Objective To screen the hemostatic active extracts from Callicarpa nudif lora .Methods The powdered Callicarpanudiflora was extracted with 70% EtOH and concentrated to give EtOH-extract .The EtOH-extract was further chromatographed over HP-20 macroporous resin column ,eluting with aqua and 95% EtOH to get HP-H2 O-elution and HP-EtOH-elution ,respectively .The obtained three extracts of EtOH-extrract ,HP-EtOH-elution and HP-H2 O-elution were set as large ,middle and small dosage groups for drug preparation ,respectively .Yunnan Baiyao was used as a positive control group . The weight increment ,bleeding time and clotting time of fed mice were detected by cutting tail and grass slide methods after in-tragastric administration for 7 days .Results As compared with blank model and positive control ,each dosage groups of HP-EtOH-elution could significantly shortened the bleeding time ,of which the small dosage group and middle dosage group even ex-hibited better results than the positive control group .Whereas the EtOH-extract ,HP-EtOH-elution and HP-H2 O-elution didn′t demonstrated significant effect on clotting time as well as the weight increment .Conclusion The HP-EtOH-elution was suggested to be the major active extract possessing hemostatic activity to mice under tested dosages .The hemostatic mechanism may through in-trinsic coagulation pathway .This study would be helpful for further phytochemical investigation of C .nudi f lora .