CAJ | 학술논문

目的：研究杂色蛤水提醇沉上清液中核苷类成分的纯化工艺。方法以 HPLC 法测定核苷含量，比较了8种型号大孔树脂对醇沉上清液中核苷类成分的纯化性质，进一步考察总核苷类成分的纯化参数，并优化纯化工艺。结果确定以 SP207大孔树脂用于纯化水提醇沉上清液中核苷类成分，其最佳工艺为：上样浓度5．71 mg／mL，上样流速1 mL／min，上样液 pH5．9，最大上样量1．6 BV，径高比为1∶10，上样后用5倍柱体积（BV）的水洗脱，再用10 BV 的5％乙醇洗脱，收集5％醇洗液，最后以水饱和的正丁醇萃取醇洗液，纯化后 HPLC 法测定11种核苷含量达61％。结论该工艺可较好地富集核苷类成分，且操作简单，稳定可行，重复性好，适合核苷类成分的纯化。
목적：연구잡색합수제순침상청액중핵감류성분적순화공예。방법이 HPLC 법측정핵감함량，비교료8충형호대공수지대순침상청액중핵감류성분적순화성질，진일보고찰총핵감류성분적순화삼수，병우화순화공예。결과학정이 SP207대공수지용우순화수제순침상청액중핵감류성분，기최가공예위：상양농도5．71 mg／mL，상양류속1 mL／min，상양액 pH5．9，최대상양량1．6 BV，경고비위1∶10，상양후용5배주체적（BV）적수세탈，재용10 BV 적5％을순세탈，수집5％순세액，최후이수포화적정정순췌취순세액，순화후 HPLC 법측정11충핵감함량체61％。결론해공예가교호지부집핵감류성분，차조작간단，은정가행，중복성호，괄합핵감류성분적순화。
ABSTRACT:OBJECTIVE To investigate the purification method on total nucleoside and nucleobases from extraction of etha-nol subsiding method from Ruditapes philippinarum .METHODS 8 types of macroporous asorption resins were used for the purification of total nucleoside from Ruditapes philippinarum extraction.HPLC was applied to determine the content of total nucleoside.Finally,SP207 macroporous asorption resin was chosen to separate the total nucleoside and nucleobases,further-more,several influence factors of purification technology were investigated.RESULTS The optimized condition of SP207 macroporous asorption resins on total nucleoside and nucleobases was as follows:sample concentration of 5.71 mg/mL,ad-sorption velocity of 1 mL/min,the sample solution of pH5.9,the maximum sample volume of 1.6 BV,the ratio of diameter to length of 1∶10,eluted with 5 BV of distilled water and 10 BV of 5% ethanol,collected eluent of 5% ethanol,and finally the 5% ethanol eluent was extracted by the n-butanol saturated with water,total nucleotide and nucleobases content after purifica-tion reached 61%.CONCLUSION This process is easy to purify nucleoside from Ruditapes philippinarum extraction,and show good,stability,feasibility and repeatability.This process could be used for the purification of total nucleoside.