CAJ | 학술논문

目的：运用代谢组学方法阐明经典激活型（M 1型）、选择活化型（M 2型）和静息态小胶质细胞的代谢差异。方法将体外培养小鼠小胶质细胞系（BV2）细胞，分为M1组、M2组和静息态组，用实时荧光定量聚合酶链反应（qRT-PCR）检测特异性mRNA的表达差异以确定细胞极化状态，采用基于气相色谱-质谱联用（GC-MS）技术的代谢组学方法阐明代谢变化。结果发现M 1型与静息态细胞的差异代谢物15个，M 2型与静息态细胞的差异代谢物15个。结论通过代谢组学方法可以找到小胶质细胞极化的差异代谢物，并解释其可能的极化机制，为神经退行性疾病的防治提供了理论依据。
목적：운용대사조학방법천명경전격활형（M 1형）、선택활화형（M 2형）화정식태소효질세포적대사차이。방법장체외배양소서소효질세포계（BV2）세포，분위M1조、M2조화정식태조，용실시형광정량취합매련반응（qRT-PCR）검측특이성mRNA적표체차이이학정세포겁화상태，채용기우기상색보-질보련용（GC-MS）기술적대사조학방법천명대사변화。결과발현M 1형여정식태세포적차이대사물15개，M 2형여정식태세포적차이대사물15개。결론통과대사조학방법가이조도소효질세포겁화적차이대사물，병해석기가능적겁화궤제，위신경퇴행성질병적방치제공료이론의거。
Objective To analyze the different metabolites of the classical activated (M1) ,alternatively activated (M2) and resting BV2 cells by metabolomics method .Methods The mRNAs of several potential biomarkers were determined by real-time PCR analyses to confirm the state of BV2 cells .Static GC-MS combined with metabolomics technology was used to analyze the metabolic changes .Results There were 15 biomarkers identified between the M1 group and the resting group ,and 15 biomarkers were found in the M2 group and the resting group .Conclusion The present study provides an effective way to reveal the mechanism of the polarization of BV 2 cell ,and it might provide a theoretical basis to prevent or treat the neurodegen-erative diseases .