CAJ | 학술논문

目的：通过纯化反应原料之一胆甾醇琥珀酸酯（CHS），提高疏水改性白及多糖的胆甾醇基取代度。方法利用重结晶法纯化CHS ，并采用差示量热扫描法（DSC）及X射线衍射法（XRD）对CHS纯品进行表征；用高效液相色谱法（HPLC）测定纯化前后产品中游离胆甾醇的含量；1 H-NMR 法测定 CHS 纯化前后改性白及多糖的胆甾醇基取代度。结果重结晶后的CHS纯度较高，具有晶体性质，可明显提高改性白及多糖的胆甾醇基取代度。结论纯化方法简便、可行，为提高改性白及多糖胆甾醇基取代度提供研究基础。
목적：통과순화반응원료지일담치순호박산지（CHS），제고소수개성백급다당적담치순기취대도。방법이용중결정법순화CHS ，병채용차시량열소묘법（DSC）급X사선연사법（XRD）대CHS순품진행표정；용고효액상색보법（HPLC）측정순화전후산품중유리담치순적함량；1 H-NMR 법측정 CHS 순화전후개성백급다당적담치순기취대도。결과중결정후적CHS순도교고，구유정체성질，가명현제고개성백급다당적담치순기취대도。결론순화방법간편、가행，위제고개성백급다당담치순기취대도제공연구기출。
Objective To improve the degree of substitution of the cholesteryl by purifying cholesteryl succinyl ester (CHS) ,one of the raw materials .Methods CHS was purified by recrystallization ,the pure CHS structure was characterizate by differential scanning calorimetry (DSC) and X ray diffraction (XRD) ,the free cholesterol content of the pure and crude were determinated by high performance liquid chromatography (HPLC) ,the degree of substitution of the cholesteryl was determina-ted before and after the purified by 1 H-NMR .Result CHS was purer after recrystallization ,with property of crystal ,which could significantly improve the degree of substitution of the cholesteryl .Conclusion The method of purification was simple and feasible ,which was the basis of improving hydrophobically modified Bletilla striata polysaccharide′s degree of substitution of the cholesteryl .