CAJ | 학술논문

目的：基于代谢组学方法研究咪康唑作用前后白念珠菌的代谢谱差异，寻找潜在的生物标志物，探讨药物作用机制。方法采用气相色谱-质谱方法测定咪康唑给药前后白念珠菌的代谢特征谱，利用多元统计分析方法进行比较，筛选潜在的生物标志物，并探讨代谢物产生差异的原因。结果经分析，筛选出23个潜在的生物标志物，主要参与了氨基酸代谢、三羧酸循环、氧化应激、糖酵解和磷脂代谢等代谢路径。结论咪康唑通过影响多种代谢路径而发挥抗真菌活性。
목적：기우대사조학방법연구미강서작용전후백념주균적대사보차이，심조잠재적생물표지물，탐토약물작용궤제。방법채용기상색보-질보방법측정미강서급약전후백념주균적대사특정보，이용다원통계분석방법진행비교，사선잠재적생물표지물，병탐토대사물산생차이적원인。결과경분석，사선출23개잠재적생물표지물，주요삼여료안기산대사、삼최산순배、양화응격、당효해화린지대사등대사로경。결론미강서통과영향다충대사로경이발휘항진균활성。
Objective To investigate the metabolism in miconazole-treated Candida albicans ,search for possible biomar-kers and discuss the mechanism of miconazole .Methods GC-MS was employed to determine the metabolic fingerprint of Can-dida albicans before and after treatment with miconazole ,and the difference based on multivariate was compared by statistical analysis ,the potential biomarkers were screened out and the mechanism of miconazole was discussed .Results Twenty three metabolites was screened out as potential biomarkers ,and they were primarily involved in amino acid metabolism ,citrate cycle , glycolysis and lipid metabolism .Conclusion The antifungal activity of miconazole was played by affecting a variety of metabolic pathways .