CAJ | 학술논문

目的：检测姜黄素诱导肝癌细胞凋亡中 lncRNA 表达谱的改变及关键 lncRNA 的筛选鉴定。方法使用不同浓度姜黄素刺激肝癌细胞株 HepG2不同时间后，提取总 RNA 进行 lncRNA 芯片杂交检测 lncRNA 表达谱的改变，筛选出差异表达 ln-cRNA；并利用 MTT 法和 TUNEL 染色观察 HepG2细胞的凋亡，Real-time PCR 验证差异表达的 lncRNA AK125910在其中的作用。结果与对照组细胞相比，姜黄素处理后的肝癌细胞中有5432条 lncRNA 表达出现改变，而其中变化倍数大于3的lncRNA 有8条，表达差异化最显著的是 lncRNA AK058003，上调7．62倍。在姜黄素诱导肝癌细胞凋亡中，lncRNA AK058003表达上调7．16倍，与芯片杂交结果基本一致。结论姜黄素诱导肝癌细胞凋亡过程中 lncRNA 表达谱出现显著变化，其中差异性表达最显著的 lncRNA AK058003可能参与了肝癌细胞的凋亡进程。
목적：검측강황소유도간암세포조망중 lncRNA 표체보적개변급관건 lncRNA 적사선감정。방법사용불동농도강황소자격간암세포주 HepG2불동시간후，제취총 RNA 진행 lncRNA 심편잡교검측 lncRNA 표체보적개변，사선출차이표체 ln-cRNA；병이용 MTT 법화 TUNEL 염색관찰 HepG2세포적조망，Real-time PCR 험증차이표체적 lncRNA AK125910재기중적작용。결과여대조조세포상비，강황소처리후적간암세포중유5432조 lncRNA 표체출현개변，이기중변화배수대우3적lncRNA 유8조，표체차이화최현저적시 lncRNA AK058003，상조7．62배。재강황소유도간암세포조망중，lncRNA AK058003표체상조7．16배，여심편잡교결과기본일치。결론강황소유도간암세포조망과정중 lncRNA 표체보출현현저변화，기중차이성표체최현저적 lncRNA AK058003가능삼여료간암세포적조망진정。
ABSTRACT:OBJECTIVE To detect the apoptosis of liver cancer cell induced by curcumin and related lncRNA expression changes and key lncRNA spectrum screening.METHODS After using different concentrations of curcumin to stimulate HepG2 cells at different times,total RNA was extracted to determine the changes of ncRNA microarray expression profiling ;and cell apoptosis of HepG2 cells was measured by using MTT assay and TUNEL staining.Real-time PCR was performed to examine the differentially expressed lncRNA AK125910.RESULTS Compared with control cells,5432 lncRNA expression in HepG2 cells induced by curcumin were changed,in which eight lncRNAs showed fold change> 3,and the most significant ex-pression differences is lncRNA AK058003 by up-regulation of 7.62 times.Curcumin induced apoptosis in liver cancer and ln-cRNA AK058003 was upregulated by 7.16-fold,consistent with the results of microarray.CONCLUSION The lncRNA ex-pression profiling in curcumin-induced apoptosis of liver cancer was significant changed.Moreover,most significant differenti-ally expressed lncRNA AK058003 may be involved in apoptosis of liver cancer cells.