CAJ | 학술논문

目的：观察山茱萸环烯醚萜苷类提取物对3T3-L1成熟脂肪细胞葡萄糖消耗及异丙肾上腺素（ISO）诱导脂肪分解的影响，探讨其分子机制。方法葡萄糖氧化酶法测定0．04、0．2、1 g／L 浓度山茱萸提取物干预24 h 后对脂肪细胞葡萄糖消耗量的影响；比色法测定干预2 d 后 ISO 30 min 诱导脂肪细胞脂肪分解的甘油释放量；Real-time PCR 法检测干预2 d 后脂肪细胞AMPK、GLUT4和 HSL mRNA 表达水平，Western blot 检测药物对 AMPK、ACC 蛋白磷酸化的影响。结果1 g／L 山茱萸提取物干预成熟脂肪细胞24 h 后可增加葡萄糖消耗，干预2 d 后可明显抑制不同浓度 ISO 诱导的脂肪分解，0．2、0．04 g／L 提取物可抑制低浓度 ISO 诱导的脂肪分解；各浓度提取物干预2 d 可不同程度上调 AMPK、GLUT4 mRNA 水平，下调 HSL mRNA 表达，1 g／L 浓度时 AMPK、ACC 磷酸化水平升高。结论山茱萸提取物可能通过上调 AMPK 基因表达的相关途径增加3T3-L1脂肪细胞葡萄糖消耗并抑制 ISO 诱导的脂肪分解。
목적：관찰산수유배희미첩감류제취물대3T3-L1성숙지방세포포도당소모급이병신상선소（ISO）유도지방분해적영향，탐토기분자궤제。방법포도당양화매법측정0．04、0．2、1 g／L 농도산수유제취물간예24 h 후대지방세포포도당소모량적영향；비색법측정간예2 d 후 ISO 30 min 유도지방세포지방분해적감유석방량；Real-time PCR 법검측간예2 d 후지방세포AMPK、GLUT4화 HSL mRNA 표체수평，Western blot 검측약물대 AMPK、ACC 단백린산화적영향。결과1 g／L 산수유제취물간예성숙지방세포24 h 후가증가포도당소모，간예2 d 후가명현억제불동농도 ISO 유도적지방분해，0．2、0．04 g／L 제취물가억제저농도 ISO 유도적지방분해；각농도제취물간예2 d 가불동정도상조 AMPK、GLUT4 mRNA 수평，하조 HSL mRNA 표체，1 g／L 농도시 AMPK、ACC 린산화수평승고。결론산수유제취물가능통과상조 AMPK 기인표체적상관도경증가3T3-L1지방세포포도당소모병억제 ISO 유도적지방분해。
ABSTRACT:OBJECTIVE To observe the effects of iridoid glycoside extract from Corni Fructus(ECO)on glucose consump-tion and isoproterenol (ISO)-induced lipolysis in 3T3-L1 mature adipocytes and possible mechanism.METHODS Glucose consumption of mature adipocytes intervened by ECO for 24 h was detected in GOP-POD method.After 2 d of ECO interven-tion,colorimetric method was used to evaluate the release amount of glycerol from 30 min ISO-induced lipolysis.The expres-sion of AMPK,GLUT4 and HSL mRNA were detected by Real-time PCR and phosphorylation levels of AMPK and ACC were evaluated by immunoblot.RESULTS In mature 3T3-L1 adipocytes,1 g/L ECO intervention increased glucose consumption and reduced the glycerol release amout from lipolysis induced by ISO with different concentrations.0.2 g/L and 0.04 g/L ECO decreased 100 nmol/L ISO-induced lipolysis.After 2 d intervention,ECO upregulated the levels of AMPK and GLUT4 mRNA and decreased the gene expression of HSL.1 g/L ECO promoted the phosphorylation of AMPK and ACC.CONCLUSION ECO can increase glucose consumption and inhibited ISO-induced lipolysis in 3T3-L1 mature adipocytes and the effects might be related to AMPK regulation.