CAJ | 학술논문

目的：通过建立炎症相关性结直肠癌小鼠模型，探讨吴茱萸碱在炎症相关性结直肠癌中的预防作用。方法用 AOM／DSS 建立炎症相关性结直肠癌模型，将小鼠分为空白组、模型组、吴茱萸碱低剂量组（10 mg／kg）和吴茱萸碱高剂量组（20 mg／kg），给药过程中记录各组小鼠体质量、死亡情况，比较各组小鼠结直肠的长度、肿瘤数目及大小，通过 HE 染色观察各组小鼠的结直肠组织形态，通过免疫组化法检测 COX-2和 PCNA 的表达。结果 AOM／DSS 可造成小鼠的体质量下降和死亡率增多，而吴茱萸碱可改善这一现象，并且减少结直肠肿瘤的发生，降低组织炎症水平和细胞的异常增殖。结论吴茱萸碱对炎症相关性结直肠癌具有良好的预防作用，其作用可能与降低组织炎症水平和抑制细胞异常增殖有关。
목적：통과건립염증상관성결직장암소서모형，탐토오수유감재염증상관성결직장암중적예방작용。방법용 AOM／DSS 건립염증상관성결직장암모형，장소서분위공백조、모형조、오수유감저제량조（10 mg／kg）화오수유감고제량조（20 mg／kg），급약과정중기록각조소서체질량、사망정황，비교각조소서결직장적장도、종류수목급대소，통과 HE 염색관찰각조소서적결직장조직형태，통과면역조화법검측 COX-2화 PCNA 적표체。결과 AOM／DSS 가조성소서적체질량하강화사망솔증다，이오수유감가개선저일현상，병차감소결직장종류적발생，강저조직염증수평화세포적이상증식。결론오수유감대염증상관성결직장암구유량호적예방작용，기작용가능여강저조직염증수평화억제세포이상증식유관。
ABSTRACT:OBJECTIVE To explore the preventive effect of evodiamine on inflammation associated colorectal carcinoma through the establishment of model in mice.METHODS The mice were administrated with AOM/DSS and divided into con-trol group,model group,low dose of evodiamine group(10EV)and high dose of evodiamine group(20EV).Body weights and death rates of mice were recorded once a week.The length of colorectum,tumor number and sizes were compared among all groups.Morphology of colorectal tissues in each group were stained by HE.Immunohistochemical analysis was used to detect the expression of COX-2 and PCNA.RESULTS AOM/DSS caused the loss of body weight and increased mortality of mice, while evodiamine improved the condition,and also reduced the development of colorectal cancer,alleviated the inflammatory response and inhibited abnormal proliferation of cancer cells.CONCLUSION Evodiamine can display promising preventive effects on inflammation-associated colorectal cancer,which is relative to the reduction of inflammation and abnormal cell prolif-eration.