南京中医药大学学报
南京中醫藥大學學報
남경중의약대학학보
JOURNAL OF NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
2015年
3期
210-213
,共4页
单核苷酸多态性%激素性股骨头坏死%基因型%证候
單覈苷痠多態性%激素性股骨頭壞死%基因型%證候
단핵감산다태성%격소성고골두배사%기인형%증후
single-nucleotide polymorphism%steroid-induced osteonecrosis of the femoral head%genotype%syndromes of Chi-nese medicine
目的：探讨 CYP1A2基因的单核苷酸多态性（SNPs）与激素性股骨头坏死中医证候的关联。方法运用聚合酶链反应-连接酶检测技术，检测70例激素性股骨头坏死患者 CYP1A2基因 T5347C、G2964A、C733A 的多态性，分析其基因型与激素性股骨头坏死中医证候之间的关联。结果 CYP1A2 T5347C 位点的基因型在2个中医证候间 TT 与 CT＋CC 基因型的分布差异无统计学意义（P ＞0．05）。CYP1A2 G2964A 位点的基因型在筋脉瘀滞证和肝肾亏损证2个中医证候间 AA 与 AG＋GG基因型的分布差异有统计学意义（P ＝0．016＜0．05，OR＝5．303）。CYP1A2 C733A 位点的基因型在2个中医证候间 CC 与 AC＋AA 基因型的分布差异无统计学意义（P＞0．05）。结论 CYP1A2 G2964A 位点 AG＋GG 基因型可能是激素性股骨头坏死患者更容易形成筋脉瘀滞证的物质基础；具有突变 AA 基因型为肝肾亏损证的危险因素。
目的：探討 CYP1A2基因的單覈苷痠多態性（SNPs）與激素性股骨頭壞死中醫證候的關聯。方法運用聚閤酶鏈反應-連接酶檢測技術，檢測70例激素性股骨頭壞死患者 CYP1A2基因 T5347C、G2964A、C733A 的多態性，分析其基因型與激素性股骨頭壞死中醫證候之間的關聯。結果 CYP1A2 T5347C 位點的基因型在2箇中醫證候間 TT 與 CT＋CC 基因型的分佈差異無統計學意義（P ＞0．05）。CYP1A2 G2964A 位點的基因型在觔脈瘀滯證和肝腎虧損證2箇中醫證候間 AA 與 AG＋GG基因型的分佈差異有統計學意義（P ＝0．016＜0．05，OR＝5．303）。CYP1A2 C733A 位點的基因型在2箇中醫證候間 CC 與 AC＋AA 基因型的分佈差異無統計學意義（P＞0．05）。結論 CYP1A2 G2964A 位點 AG＋GG 基因型可能是激素性股骨頭壞死患者更容易形成觔脈瘀滯證的物質基礎；具有突變 AA 基因型為肝腎虧損證的危險因素。
목적：탐토 CYP1A2기인적단핵감산다태성（SNPs）여격소성고골두배사중의증후적관련。방법운용취합매련반응-련접매검측기술，검측70례격소성고골두배사환자 CYP1A2기인 T5347C、G2964A、C733A 적다태성，분석기기인형여격소성고골두배사중의증후지간적관련。결과 CYP1A2 T5347C 위점적기인형재2개중의증후간 TT 여 CT＋CC 기인형적분포차이무통계학의의（P ＞0．05）。CYP1A2 G2964A 위점적기인형재근맥어체증화간신우손증2개중의증후간 AA 여 AG＋GG기인형적분포차이유통계학의의（P ＝0．016＜0．05，OR＝5．303）。CYP1A2 C733A 위점적기인형재2개중의증후간 CC 여 AC＋AA 기인형적분포차이무통계학의의（P＞0．05）。결론 CYP1A2 G2964A 위점 AG＋GG 기인형가능시격소성고골두배사환자경용역형성근맥어체증적물질기출；구유돌변 AA 기인형위간신우손증적위험인소。
ABSTRACT:OBJECTIVE To investigate the relationship between single nucleotide polymorphisms of CYP1A2 gene and TCM syndrome of steroid-induced osteonecrosis of the femoral head(SONFH).METHODS By using polymerase chain reac-tion-ligase detection technology,this paper detected polymorphism of T5347C,G2964A and C733A in the CYP1A2 gene of 70 SONFH cases,and analysed the relationship between genotype and TCM syndrome of this kind of SONFH.RESULTS The comparison of distribution of TT and CT+CC genotypes in CYP1A2 T5347C sites between two syndromes had no statistical significance(P >0.05).The difference of AA and AG + GG genotypes in CYP1A2 G2964A sites between the two syndromes was statistically significant(P <0.05,OR=5.303).The difference of CC and AC+AA genotypes in CYP1A2 C733A sites be-tween the two syndromes was not statistically significant(P >0.05).CONCLUSION The site of CYP1A2 G2964A in AG+GG genotype may be the material basis of the stasis in tendons and vessels Syndrome in the patients with SONFH.Genotype of mutated AA may be the risk factor of liver and kidney deficiency syndrome.
