CAJ | 학술논문

目的：初步评价单一位点夹心抗体（SES）法结合循环增强荧光免疫技术（CEIFA）检测 B 型钠尿肽（BNP）的性能。方法用 SES 法结合 CEIFA 仪检测 BNP，对其精密度、线性范围、功能敏感性、回收率、样本稳定性及与西门子 ADVIA Centaur CP 化学发光仪（简称 Centaur CP）的相关性进行评估。结果 SES 法结合 CEIFA的批内和批间变异系数（CV）分别为4．38％～7．84％和10．47％～11．21％。线性范围为25～5000 pg／mL。功能敏感性为12．5 pg／mL。回收率为95．04％～109．2％。与 Centaur CP 比较，其回归方程为 Y ＝0．9983X －22．38（r ＝0．969，P ＜0．01）。对样本稳定性的要求低于 Centaur CP。结论 SES 法结合 CEIFA 测定 BNP 具有较好的性能，与传统的双抗体夹心发光法比较有一定优势，适合临床急诊实验室使用。
목적：초보평개단일위점협심항체（SES）법결합순배증강형광면역기술（CEIFA）검측 B 형납뇨태（BNP）적성능。방법용 SES 법결합 CEIFA 의검측 BNP，대기정밀도、선성범위、공능민감성、회수솔、양본은정성급여서문자 ADVIA Centaur CP 화학발광의（간칭 Centaur CP）적상관성진행평고。결과 SES 법결합 CEIFA적비내화비간변이계수（CV）분별위4．38％～7．84％화10．47％～11．21％。선성범위위25～5000 pg／mL。공능민감성위12．5 pg／mL。회수솔위95．04％～109．2％。여 Centaur CP 비교，기회귀방정위 Y ＝0．9983X －22．38（r ＝0．969，P ＜0．01）。대양본은정성적요구저우 Centaur CP。결론 SES 법결합 CEIFA 측정 BNP 구유교호적성능，여전통적쌍항체협심발광법비교유일정우세，괄합림상급진실험실사용。
Objective To evaluate B-type natriuretic peptide quantification by single-epitope sandwich ( SES) combined with cyclic enhanced immunofluorescent assay(CEIFA).Methods By SES combined with CEIFA, BNP was determined.The precision, linear range, function sensitivity, recovery rate and sample stability were evaluated, and 45 samples were determined by the method, which the results were evaluated with those of SIEMENS ADVIA Centaur CP chemiluminescence analyzer (Centaur CP).Results The within-run and between-run coefficients of variation (CV) were 4.38%-7.84% and 10.47%-11.21%, respectively.The linear range was 25-5 000 pg/mL.The function sensitivity was 12.5 pg/mL.The recovery rate was 95.04%-109.2%.The correlation formula between the novel method and Centaur CP was Y =0.998 3X -22.38 (r =0.969, P <0.01).The request of sample stability of SES combined with CEIFA was lower than that of Centaur CP.Conclusions SES combined with CEIFA for BNP quantification has a good performance, and there are some advantages compared with traditional double-antibody sandwich chemiluminescence assay.