CAJ | 학술논문

目的：探讨子宫内膜异位症与 Fas 介导的腹腔液单个核细胞凋亡的关系。方法选取手术后病理证实为子宫内膜异位症的患者55例作为子宫内膜异位症组，以非子宫内膜异位症患者52例作为对照组，分离腹腔液单个核细胞，应用实时逆转录聚合酶链反应（RT－PCR）测定 Fas mRNA 的表达水平，应用流式细胞术定量检测 Fas 表达细胞百分率。结果子宫内膜异位症组腹腔液单个核细胞 Fas mRNA 及 Fas 表达细胞百分率明显高于对照组（P ＜0．05），Fas mRNA 及 Fas 表达细胞百分率在子宫内膜异位症组Ⅰ～Ⅱ期与Ⅲ～Ⅳ期中表达差异无统计学意义（P ＞0．05）。结论子宫内膜异位症患者腹腔液单个核细胞 Fas 表达异常，对子宫内膜异位症具有一定的作用。
목적：탐토자궁내막이위증여 Fas 개도적복강액단개핵세포조망적관계。방법선취수술후병리증실위자궁내막이위증적환자55례작위자궁내막이위증조，이비자궁내막이위증환자52례작위대조조，분리복강액단개핵세포，응용실시역전록취합매련반응（RT－PCR）측정 Fas mRNA 적표체수평，응용류식세포술정량검측 Fas 표체세포백분솔。결과자궁내막이위증조복강액단개핵세포 Fas mRNA 급 Fas 표체세포백분솔명현고우대조조（P ＜0．05），Fas mRNA 급 Fas 표체세포백분솔재자궁내막이위증조Ⅰ～Ⅱ기여Ⅲ～Ⅳ기중표체차이무통계학의의（P ＞0．05）。결론자궁내막이위증환자복강액단개핵세포 Fas 표체이상，대자궁내막이위증구유일정적작용。
Objective To investigate the relationship of Fas expression in peritoneal fluid mononuclear cells and endometriosis.Methods A total of 55 patients with histopathologically diagnosed endometriosis after operation were enrolled as endometriosis group, and 52 patients without endometriosis were enrolled as control group.The peritoneal fluid mononuclear cells were isolated, the expression levels of Fas mRNA were determined by reverse transcriptase-polymerase chain reaction (RT-PCR), and the percentage of Fas expression cells were quantitatively detected by flow cytometry.Results Fas mRNA and the percentage of Fas expression cells were significantly higher in endometriosis group than those in control group(P <0.05).There was no statistical significance between stage Ⅰ-Ⅱand stage Ⅲ-Ⅳ(P >0.05).Conclusions The abnormal Fas expression in peritoneal fluid mononuclear cells may play an important role in endometriosis.