现代中西医结合杂志
現代中西醫結閤雜誌
현대중서의결합잡지
MODERN JOURNAL OF INTEGRATED TRADITIONAL CHINESE AND WESTERN MEDICINE
2015年
13期
1384-1387
,共4页
李琦军%吴永波%常军英%侯卫星%邢兆国%王彦志%穆卫庐%李炎%贾东昭%张淑丽
李琦軍%吳永波%常軍英%侯衛星%邢兆國%王彥誌%穆衛廬%李炎%賈東昭%張淑麗
리기군%오영파%상군영%후위성%형조국%왕언지%목위려%리염%가동소%장숙려
神经肽-Y%小神经胶质细胞%肿瘤坏死因子-α
神經肽-Y%小神經膠質細胞%腫瘤壞死因子-α
신경태-Y%소신경효질세포%종류배사인자-α
neuropeptide Y%microglia%TNF-α
目的：探讨神经肽Y（ NPY）对原代小神经胶质细胞生物活性及生成肿瘤坏死因子－α（ TNF－α）的影响。方法培养原代大鼠皮质小胶质细胞，经脂多糖（ LPS）和NPY处理后行免疫细胞化学荧光染色，显微镜下观察LPS和NPY对小胶质细胞形态学的影响。将小胶质细胞分为对照组、LPS组、NPY＋LPS组、NPY组和BIBP3226＋NPY＋LPS组，每组3个样本，培养各组细胞6 h。 ELISA法检测培养液中TNF－α蛋白含量，RT－PCR方法检测小胶质细胞中TNF－αmRNA表达水平。结果 LPS处理后小胶质细胞处于活化状态，NPY处理后的小胶质细胞活化水平降低。 LPS组和IBP 3226＋NPY＋LPS组培养液中TNF－α蛋白含量及细胞中TNF－αmRNA表达水平显著高于对照组（P均＜0．05）；LPS＋NPY组TNF－α蛋白含量和TNF－αmRNA表达水平明显低于LPS组和IBP3226＋NPY＋LPS组（ P均＜0．05）。结论 NPY能够降低小神经胶质细胞的生物活性。 NPY可能通过激活NPY Y1受体抑制小神经胶质细胞生成TNF－α。
目的：探討神經肽Y（ NPY）對原代小神經膠質細胞生物活性及生成腫瘤壞死因子－α（ TNF－α）的影響。方法培養原代大鼠皮質小膠質細胞，經脂多糖（ LPS）和NPY處理後行免疫細胞化學熒光染色，顯微鏡下觀察LPS和NPY對小膠質細胞形態學的影響。將小膠質細胞分為對照組、LPS組、NPY＋LPS組、NPY組和BIBP3226＋NPY＋LPS組，每組3箇樣本，培養各組細胞6 h。 ELISA法檢測培養液中TNF－α蛋白含量，RT－PCR方法檢測小膠質細胞中TNF－αmRNA錶達水平。結果 LPS處理後小膠質細胞處于活化狀態，NPY處理後的小膠質細胞活化水平降低。 LPS組和IBP 3226＋NPY＋LPS組培養液中TNF－α蛋白含量及細胞中TNF－αmRNA錶達水平顯著高于對照組（P均＜0．05）；LPS＋NPY組TNF－α蛋白含量和TNF－αmRNA錶達水平明顯低于LPS組和IBP3226＋NPY＋LPS組（ P均＜0．05）。結論 NPY能夠降低小神經膠質細胞的生物活性。 NPY可能通過激活NPY Y1受體抑製小神經膠質細胞生成TNF－α。
목적：탐토신경태Y（ NPY）대원대소신경효질세포생물활성급생성종류배사인자－α（ TNF－α）적영향。방법배양원대대서피질소효질세포，경지다당（ LPS）화NPY처리후행면역세포화학형광염색，현미경하관찰LPS화NPY대소효질세포형태학적영향。장소효질세포분위대조조、LPS조、NPY＋LPS조、NPY조화BIBP3226＋NPY＋LPS조，매조3개양본，배양각조세포6 h。 ELISA법검측배양액중TNF－α단백함량，RT－PCR방법검측소효질세포중TNF－αmRNA표체수평。결과 LPS처리후소효질세포처우활화상태，NPY처리후적소효질세포활화수평강저。 LPS조화IBP 3226＋NPY＋LPS조배양액중TNF－α단백함량급세포중TNF－αmRNA표체수평현저고우대조조（P균＜0．05）；LPS＋NPY조TNF－α단백함량화TNF－αmRNA표체수평명현저우LPS조화IBP3226＋NPY＋LPS조（ P균＜0．05）。결론 NPY능구강저소신경효질세포적생물활성。 NPY가능통과격활NPY Y1수체억제소신경효질세포생성TNF－α。
Objective It is to explore the effect of NPY on biological activity of primary microglia and the production of TNF-ɑin the rats.Methods Primary cerebral cortical microglia were cultured and treated with LPS and NPY .Then they were stained by immunocytochemistry staining and the morphological characteristics of microglia was observed with microscope . The microglia were divided into control group , LPS group, NPY+LPS group, NPY group and BIBP3226+NPY+LPS group. Every group had 3 samples and the the cells were cultured for 6 h.The protein levels of TNF-ɑin the culture media and the mRNA expression leves of TNF -ɑin the microglia cells of different groups were detected with the methods of ELISA and RT -PCR respectively .Results The microglia treated with LPS kept active , the activity of microglias treated with NPY was lower . The contents of TNF-ɑin the culture media and the mRNA expression levels of TNF -ɑin the cells of LPS group increased remarkably compared with control group (P<0.05).Compared with LPS group, the contents of TNF-ɑin the culture media and the mRNA expression levels of TNF -ɑ in the cells of LPS +NPY group reduced obviously .Compared with LPS +NPY group, the contents of TNF-ɑin the culture media and the mRNA expression levels of TNF -ɑ in the cells of BIBP3226+NPY+LPS group rose outstandingly (P<0.05).Conclusion NPY can restrain the biological activity of microglia cells .NPY can reduce the production of TNF -ɑof microglia cells , Activation of NPY Y1 recepter on the microglia cells may be one of the reasons .
