CAJ | 학술논문

目的：通过小鼠模型试验，探讨姜黄素对肥胖相关性肾小球病（ ORG）的防治作用。方法：21只C57BL／6J雄性小鼠被随机分为对照组、ORG模型组及姜黄素干预组进行试验。在光镜下用计算机图像分析软件测量肾小球直径，用实时定量聚合酶链反应和Western印迹试验分别检测mRNA及蛋白表达。结果：与对照组相比，模型组小鼠体重、Lee指数、腹腔脂肪指数、尿蛋白定量、肾小球平均直径均显著增加；nephrin、podocin、podoplanin、podocalyxin的mRNA和蛋白表达均显著降低；肾组织Wnt1、Wnt2b、Wnt6及β-catenin的mRNA表达均显著上调；β-catenin蛋白的磷酸化水平显著下降。而姜黄素干预能够有效拮抗上述全部反应。结论：姜黄素能通过抑制Wnt／β-catenin信号通路激活而减轻肥胖及其肾损伤。
목적：통과소서모형시험，탐토강황소대비반상관성신소구병（ ORG）적방치작용。방법：21지C57BL／6J웅성소서피수궤분위대조조、ORG모형조급강황소간예조진행시험。재광경하용계산궤도상분석연건측량신소구직경，용실시정량취합매련반응화Western인적시험분별검측mRNA급단백표체。결과：여대조조상비，모형조소서체중、Lee지수、복강지방지수、뇨단백정량、신소구평균직경균현저증가；nephrin、podocin、podoplanin、podocalyxin적mRNA화단백표체균현저강저；신조직Wnt1、Wnt2b、Wnt6급β-catenin적mRNA표체균현저상조；β-catenin단백적린산화수평현저하강。이강황소간예능구유효길항상술전부반응。결론：강황소능통과억제Wnt／β-catenin신호통로격활이감경비반급기신손상。
Objective:To study the effects of Curcumin intervention on mouse model of obesity related glomerulopathy (ORG). Methods:Twenty one male C57BL/6J mice were randomly divided into control group(CG),ORG model group(MG)and Curcumin intervention group. Glomerular diameters were measured by light microscopy. mRNA and protein expression was detected by real-time quantitative PCR and Western blotting,respectively. Results:Compared with the CG,body weight,Lee’s index,abdomi-nal fat index,urinary protein excretion,glomerular average diameter all were significantly increased in MG(P<0. 05 or 0. 01);Nephrin、podocin、podoplanin、podocalyxin mRNA and protein levels all were significantly decreased in MG( P <0. 05 );Wnt1, Wnt2b,Wnt6 andβ-catenin mRNA expression of renal tissue was significantly up-regulated and phosphorylation level ofβ-cate-nin protein was significantly reduced in MG(P<0. 05). Curcumin intervention effectively antagonized the all above reactions(P<0. 05). Conclusion:Curcumin can relieve obesity and ORG by means of inhibition of Wnt/β-catenin signaling activation.