CAJ | 학술논문

以黑龙江省正茬土壤为研究对象，采用室内回接培养的方法，研究了大豆根系分泌物中2种有机酸（苹果酸、酒石酸）和3种酚酸（油酸甲酯、2，4-二叔丁基苯酚和香草酸）对土壤微生物量和微生物活性的影响，并通过测定土壤微生物 C／N 比，初步确定有显著作用的分泌物组分。结果表明，苹果酸、酒石酸和油酸甲酯的加入对微生物来说是有益的，化感作用并没有表现出来；而2，4-二叔丁基苯酚和香草酸的加入，对土壤微生物的生长表现为低浓度促进高浓度抑制，对土壤微生物有化感作用。
이흑룡강성정치토양위연구대상，채용실내회접배양적방법，연구료대두근계분비물중2충유궤산（평과산、주석산）화3충분산（유산갑지、2，4-이숙정기분분화향초산）대토양미생물량화미생물활성적영향，병통과측정토양미생물 C／N 비，초보학정유현저작용적분비물조분。결과표명，평과산、주석산화유산갑지적가입대미생물래설시유익적，화감작용병몰유표현출래；이2，4-이숙정기분분화향초산적가입，대토양미생물적생장표현위저농도촉진고농도억제，대토양미생물유화감작용。
This study aimed to determine the relationship between allelopathy and soil microorganisms in order to find out the possible barriers that inhibit continuous cropping of soybeans.By altering two organic acids (i.e.,malic and tartaric acid)and three phenolic acids (i.e.,oleic acid methyl ester,2,4-dinitrophenol and vanillic acid)among the known soybean root exudates,the effect on the biomass and activity of the microbes in soil was determined. Using the soil sample collected from the continuous cropping field in Heilongjiang province,the in-lab frankia culture method was employed and the carbon/nitrogen ratio of the soil microbial biomass measured to preliminary identify the major components in the exudate.The results indicated that the addition of malic acid,tartaric acid and oleic acid methyl ester benefitted the microbial growth without allelopathy effect.On the other hand,the addition of 2,4-dinitrophenol and vanillic acid at low concentrations increased the microbial biomass,while reduced it at high concentrations.The results suggested that 2,4-dinitrophenol and vanillic acid might play an important role in the soybean allelopathy.