肉类工业
肉類工業
육류공업
MEAT INDUSTRY
2015年
5期
32-37
,共6页
徐晓霞%张怀珠%冯晓群%彭涛%温科
徐曉霞%張懷珠%馮曉群%彭濤%溫科
서효하%장부주%풍효군%팽도%온과
中药材提取物%响应面%变形杆菌%抑菌作用
中藥材提取物%響應麵%變形桿菌%抑菌作用
중약재제취물%향응면%변형간균%억균작용
Chinese herbal medicine%responses surface%proteus vulgaris%bacteriostatic effect
在单因素实验的基础上，运用响应面法优化了甘肃丹参、黄芪、甘草、金银花4种甘肃地道中药材乙醇提取物配伍后对变形杆菌的抑制作用。结果表明中药材提取物的最优配伍浓度为：丹参0．14g／mL、黄芪0．11g／mL、甘草0．17g／mL、金银花0．07g／mL，在此条件下抑菌率实测值为57．24％，测得配伍组合的最低抑菌浓度为0?39g／mL。配伍中药材提取物对变形杆菌的抑菌圈直径达到18．5mm，与对照组的抑菌效果接近，表明对变形杆菌有较好的抑制作用。
在單因素實驗的基礎上，運用響應麵法優化瞭甘肅丹參、黃芪、甘草、金銀花4種甘肅地道中藥材乙醇提取物配伍後對變形桿菌的抑製作用。結果錶明中藥材提取物的最優配伍濃度為：丹參0．14g／mL、黃芪0．11g／mL、甘草0．17g／mL、金銀花0．07g／mL，在此條件下抑菌率實測值為57．24％，測得配伍組閤的最低抑菌濃度為0?39g／mL。配伍中藥材提取物對變形桿菌的抑菌圈直徑達到18．5mm，與對照組的抑菌效果接近，錶明對變形桿菌有較好的抑製作用。
재단인소실험적기출상，운용향응면법우화료감숙단삼、황기、감초、금은화4충감숙지도중약재을순제취물배오후대변형간균적억제작용。결과표명중약재제취물적최우배오농도위：단삼0．14g／mL、황기0．11g／mL、감초0．17g／mL、금은화0．07g／mL，재차조건하억균솔실측치위57．24％，측득배오조합적최저억균농도위0?39g／mL。배오중약재제취물대변형간균적억균권직경체도18．5mm，여대조조적억균효과접근，표명대변형간균유교호적억제작용。
Based on the single-factor experiments, the responses surface methodology( RSM) were applied to study the bacteriostatic effect of four Chinese herbal medicine of salvia, astragalus, glycyrrhiza and honeysuckle extracted by ethanol on the proteus vulgaris. The results showed that the optimal con?centration of salvia, astragalus, glycyrriza, and honeysuckle was 0. 14 g/mL, 0. 11 g/mL, 0. 17g/mL and 0. 07g/mL, respectively. Under this condition, the bacteriostatic rate was 57. 24%. The minimum inhibitory of compatibility concentration was 0. 39g/mL. And the diameter of inhibition zone was 18?5mm, closed to the control group, which indicated the bacteriostatic effect on the Proteus vulgaris was distinctly.
