CAJ | 학술논문

对水稻第1染色体长臂上控制粒重、粒长和粒宽的3个 QTLs 进行了剖析.从珍汕973/密阳46的 BC2 F8群体中筛选到1个在 RM212－RM265区间呈杂合的单株,应用 DNA 标记检测其衍生的 BC2 F9群体,筛选出在 RM212－RM11787和RM11787－RM265区间呈杂合的单株各1个,其杂合区间分别覆盖前期定位的千粒重 QTL qTGW1．2a 和 qTGW1．2b .种植2个 BC2 F10群体,筛选出杂合区间相互交叠的单株各3个,自交后获得6个 BC2 F11群体,在 RM11781－RM11800区间检测到了1个控制粒长的 QTL,并将 qTGW1．2a 和 qTGW1．2b 分别缩小至580 kb 的 RM11730－ Wn33304和2．0 Mb 的RM11807－RM11885区间内.同时,筛选出5个杂合区间相互交叠的单株,衍生了5个 BC2 F12群体.QTL 分析结果表明,每个群体均检测到粒长 QTL,加性效应为0．03~0．06 mm,增效等位基因来自密阳46；经比较各个群体的分离区间,将控制粒长的 QTL qGL1．2界定在 RM11781和 Wn34526之间372 kb 的区间内；该 QTL 呈加性作用,可能同时控制粒重、粒长与粒宽.
대수도제1염색체장비상공제립중、립장화립관적3개 QTLs 진행료부석.종진산973/밀양46적 BC2 F8군체중사선도1개재 RM212－RM265구간정잡합적단주,응용 DNA 표기검측기연생적 BC2 F9군체,사선출재 RM212－RM11787화RM11787－RM265구간정잡합적단주각1개,기잡합구간분별복개전기정위적천립중 QTL qTGW1．2a 화 qTGW1．2b .충식2개 BC2 F10군체,사선출잡합구간상호교첩적단주각3개,자교후획득6개 BC2 F11군체,재 RM11781－RM11800구간검측도료1개공제립장적 QTL,병장 qTGW1．2a 화 qTGW1．2b 분별축소지580 kb 적 RM11730－ Wn33304화2．0 Mb 적RM11807－RM11885구간내.동시,사선출5개잡합구간상호교첩적단주,연생료5개 BC2 F12군체.QTL 분석결과표명,매개군체균검측도립장 QTL,가성효응위0．03~0．06 mm,증효등위기인래자밀양46；경비교각개군체적분리구간,장공제립장적 QTL qGL1．2계정재 RM11781화 Wn34526지간372 kb 적구간내；해 QTL 정가성작용,가능동시공제립중、립장여립관.
This paper reports the dissection of three quantitative trait loci(QTLs)for 1000-grain weight (TGW),grain length(GL)and grain width (GW)on the long arm of chromosome 1 in rice.A plant carrying a heterozygous segment extending from RM212 to RM265 was selected from the Zhenshan 97 3/Milyang 46 BC2 F8 population.The resulting BC2 F9 population was assayed with DNA markers.Two plants were identified,carrying heterozygous segments covering the intervals RM212 - RM1 1 787 and RM1 1 787 - RM265 which were previously found to harbor qTGW1 .2a and qTGW1 .2b for TGW,respectively.Two BC2 F1 0 populations were produced,from which three plants with sequential heterozygous regions were identified,respectively.Using the six BC2 F1 1 populations derived,a QTL for GL was detected in the interval RM1 1 730-RM1 1800,and the regions for qTGW1 .2a and qTGW1 .2b were narrowed down to the 580 kb interval RM1 1 730-Wn33304 and 2.0 Mb interval RM1 1807-RM1 1885 ,respectively.In the meantime,five plants with smaller heterozygous regions were selected,from which five BC2 F1 2 populations were constructed.A QTL for GL was detected in each of the five populations.The additive effect ranged from 0.03 mm to 0.06 mm with the enhancing alleles derived from Milyang 46.Based on comparison of the segregating regions among the five populations,qGL1 .2 for GL was delimited to a 372 kb region flanked by RM1 1 781 and Wn34526.This QTL had the additive genetic action mode and may simultaneously affect TGW,GL and GW.