解放军医学院学报
解放軍醫學院學報
해방군의학원학보
Academic Journal of Chinese Pla Medical School
2015年
5期
482-486
,共5页
周明月%王可伊%张延明%赵云%温宏峰%孟凡磊%方伯言
週明月%王可伊%張延明%趙雲%溫宏峰%孟凡磊%方伯言
주명월%왕가이%장연명%조운%온굉봉%맹범뢰%방백언
阿尔茨海默病%基因芯片%水通道蛋白-4
阿爾茨海默病%基因芯片%水通道蛋白-4
아이자해묵병%기인심편%수통도단백-4
Alzheimer's disease%gene microarray%aquaporin 4
目的:探讨水通道蛋白-4(aquaporin 4,AQP4)基因表达与阿尔茨海默病的相关性。方法应用Agilent表达谱芯片,选用快速老化痴呆模型小鼠(SAMP8),通过与同月龄正常对照小鼠(SAMR1)比较,提取脑组织总RNA,筛选出差异基因并应用Real time-PCR技术和免疫荧光方法进行鉴定。结果筛选差异表达基因共804个,其中表达上调521个,表达下调283个,在GeneBank查找相关基因的生物学功能及通路,找到与AD密切相关基因有6条:Herc6,Lyst,Nkain1,Aqp4, Uch13,Slc9a8,其中Aqp4基因的差异倍数为6.26(P<0.001)。Real time-PCR检测结果显示,SAMP8组Aqp4基因表达量较SAMR1组增高(P=0.0376),免疫荧光提示SAMP8组中Aqp4表达增加。结论 Aqp4在SAMP8小鼠中表达量增多,可能与清除β淀粉样蛋白沉积、代偿性星形胶质细胞增生有关。
目的:探討水通道蛋白-4(aquaporin 4,AQP4)基因錶達與阿爾茨海默病的相關性。方法應用Agilent錶達譜芯片,選用快速老化癡呆模型小鼠(SAMP8),通過與同月齡正常對照小鼠(SAMR1)比較,提取腦組織總RNA,篩選齣差異基因併應用Real time-PCR技術和免疫熒光方法進行鑒定。結果篩選差異錶達基因共804箇,其中錶達上調521箇,錶達下調283箇,在GeneBank查找相關基因的生物學功能及通路,找到與AD密切相關基因有6條:Herc6,Lyst,Nkain1,Aqp4, Uch13,Slc9a8,其中Aqp4基因的差異倍數為6.26(P<0.001)。Real time-PCR檢測結果顯示,SAMP8組Aqp4基因錶達量較SAMR1組增高(P=0.0376),免疫熒光提示SAMP8組中Aqp4錶達增加。結論 Aqp4在SAMP8小鼠中錶達量增多,可能與清除β澱粉樣蛋白沉積、代償性星形膠質細胞增生有關。
목적:탐토수통도단백-4(aquaporin 4,AQP4)기인표체여아이자해묵병적상관성。방법응용Agilent표체보심편,선용쾌속노화치태모형소서(SAMP8),통과여동월령정상대조소서(SAMR1)비교,제취뇌조직총RNA,사선출차이기인병응용Real time-PCR기술화면역형광방법진행감정。결과사선차이표체기인공804개,기중표체상조521개,표체하조283개,재GeneBank사조상관기인적생물학공능급통로,조도여AD밀절상관기인유6조:Herc6,Lyst,Nkain1,Aqp4, Uch13,Slc9a8,기중Aqp4기인적차이배수위6.26(P<0.001)。Real time-PCR검측결과현시,SAMP8조Aqp4기인표체량교SAMR1조증고(P=0.0376),면역형광제시SAMP8조중Aqp4표체증가。결론 Aqp4재SAMP8소서중표체량증다,가능여청제β정분양단백침적、대상성성형효질세포증생유관。
Objective To explore the correlation between aquaporin 4 (AQP4) and Alzheimer's disease (AD).MethodsAgilent expressionprofile chip was used to screen the gene expression differences through the comparison between SAMP8 and SAMR1 after total RNA of brain tissue being extracted out, then Real-Time PCR technology and indirect immunofluorescence assay were applied for identification.ResultsTotally 804 genes related to dementia were selected, among which the number of up-regulated genes was 521 and down-regulated genes was 283. The biological function and pathways of the related genes were searched in GeneBank, then six genes were found to be closely related to AD: Herc6, Lyst, Nkain1, Aqp4, Uch13, Slc9a8. The fold change of Aqp4 was 6.26 withP<0.001. The Real-time PCR of the Aqp4 gene showed that the expression quantity in SAMP8 group was higher than that in SAMR1 group (P= 0.037 6), and the immunofluorescence assayshowed that the expression of Aqp4 in SAMP8 also increased.Conclusion Increased Aqp4 expression is related to the increased need for Aβ clearance in SAMP8 rats which has astrocytes compensatory proliferation.