CAJ | 학술논문

目的探讨芦荟多糖(AP)预处理对初进高原重度失血性休克大鼠海马组织核因子-κB (NF-κB)、细胞间黏附分子-1(ICAM-1)表达及海马组织细胞凋亡程度的影响.方法雄性SD大鼠40只,体质量250 ～ 300 g.随机(随机数字法)分为5组(n=8):假手术(Sham)组、休克(Shock)组和芦荟多糖预处理(AP)组,AP组按剂量分为0.75 mg/ kg (AP1)、1.50mg/kg (AP2)和3.00 mg/kg (AP3)3个亚组.Sham组仅行动静脉穿刺,不放血;Shock组仅放血,无液体复苏;AP1、AP2、AP3组分别于放血前30 min股静脉注射不同剂量AP.采用股动脉放血法制备重度失血性休克模型,15 min内使平均动脉压降至(35±5) mmHg(1 mmHg=0.133kPa),通过放血或自体血回输维持此水平60 min.于复苏后3h,放血处死大鼠,冰上取脑分离海马.采用免疫组织化学法检测海马组织NF-κB和ICAM-1的表达,并用TUNEL法检测海马组织细胞凋亡.各组间均数比较采用单因素方差分析,以P＜ 0.05为差异具有统计学意义.结果与Sham组比较,Shock组海马组织NF-κB表达水平(5.03±0.42)和ICAM-1表达水平(4.14±0.29)增高,海马组织细胞凋亡数(44.3±7.2)增加(P ＜0.05);Shock组和AP1组间上述各指标差异均无统计学意义.与Shock组比较,AP2组海马组织NF-κB表达水平(3.12±0.34)和ICAM-1表达水平(2.93 ±0.21)降低,海马组织细胞凋亡数(24.8±3.6)减少(P ＜0.05);AP2和AP3组间上述各指标差异均无统计学意义.结论芦荟多糖预处理可抑制初进高原重度失血性休克大鼠海马组织NF-κB与ICAM-1表达和减轻海马组织细胞凋亡程度.
목적 탐토호회다당(AP)예처리대초진고원중도실혈성휴극대서해마조직핵인자-κB (NF-κB)、세포간점부분자-1(ICAM-1)표체급해마조직세포조망정도적영향.방법 웅성SD대서40지,체질량250 ～ 300 g.수궤(수궤수자법)분위5조(n=8):가수술(Sham)조、휴극(Shock)조화호회다당예처리(AP)조,AP조안제량분위0.75 mg/ kg (AP1)、1.50mg/kg (AP2)화3.00 mg/kg (AP3)3개아조.Sham조부행동정맥천자,불방혈;Shock조부방혈,무액체복소;AP1、AP2、AP3조분별우방혈전30 min고정맥주사불동제량AP.채용고동맥방혈법제비중도실혈성휴극모형,15 min내사평균동맥압강지(35±5) mmHg(1 mmHg=0.133kPa),통과방혈혹자체혈회수유지차수평60 min.우복소후3h,방혈처사대서,빙상취뇌분리해마.채용면역조직화학법검측해마조직NF-κB화ICAM-1적표체,병용TUNEL법검측해마조직세포조망.각조간균수비교채용단인소방차분석,이P＜ 0.05위차이구유통계학의의.결과 여Sham조비교,Shock조해마조직NF-κB표체수평(5.03±0.42)화ICAM-1표체수평(4.14±0.29)증고,해마조직세포조망수(44.3±7.2)증가(P ＜0.05);Shock조화AP1조간상술각지표차이균무통계학의의.여Shock조비교,AP2조해마조직NF-κB표체수평(3.12±0.34)화ICAM-1표체수평(2.93 ±0.21)강저,해마조직세포조망수(24.8±3.6)감소(P ＜0.05);AP2화AP3조간상술각지표차이균무통계학의의.결론 호회다당예처리가억제초진고원중도실혈성휴극대서해마조직NF-κB여ICAM-1표체화감경해마조직세포조망정도.
Objective To investigate the effect of aloe polysaccharides (AP) pre-emptive treatment on the expression of nuclear factor kappa B (NF-κB),ntercellulor adhesion molecule-1 (ICAM-1) and cell apoptosis in hippocampal brain tissue in rats with severely hemorrhagic shock for the first time of entering high altitude.Methods Forty healthy male SD rats weighing 250-300 g were randomly (random number) divided into 5 groups (n =8 each):sham group,shock group and AP group which was further divided into 3 subgroups as per different dosages of AP administered (AP1:0.75 mg/kg; AP2:1.50 mg/kg; AP3:3.00 mg/kg).Rats in sham group were treated with surgical procedure without exsanguination.Rats in shock group were exsanguinated until hemorrhagic shock emerged without resuscitation.Rats in AP subgroups were intravenously infused with given doses of AP in different AP subgroups at 30 min before hemorrhagic shock.MAP was dropped to (35 ±5) mmHg (1 mmHg =0.133 kPa) in 15 min by bleeding from femoral artery,the mean arterial pressure (MAP) was maintained at (35 ±5) mmHg for 60 min with bleeding or re-transfusing.At 3 h after resuscitation,rats were sacrificed immediately by bleeding,and the hippocampus of brain was harvested on the ice.The expressions of NF-κB and ICAM-1 in the hippocampus of rats were determined by immuno-histochemical method,and number of cell apoptosis in the hippocampus of rats was determined by TUNEL.The means were compared with analysis of variance and Student-NewmanKeuls test,and statistical significance was established at a P value of less than 0.05.Results Compared with sham group,the expressions of NF-κB (5.03 ±0.42),ICAM-1 (4.14 ±0.29) and number of cell apoptosis (44.3 ± 7.2) in hippocampal tissue were significantly increased in shock group (P ＜ 0.05).There were no significant differences in these three variables between shock group and AP1 group.Compared with shock group,the expressions of NF-κB (3.12 ±0.34),ICAM-1 (2.93 ±0.21) and number of cell apoptosis (24.8 ± 3.6) in hippocampal tissue were significantly decreased in AP2 group (P ＜ 0.05).There were no significant differences in these three variables between AP2 and AP3 groups.Conclusion AP pre-emptive treatment can significantly attenuate the expressions of NF-κB,ICAM-1 and number of cell apoptosis in hippocampal tissue in hemorrhagic shock rats.