CAJ | 학술논문

以‘南林895’杨（Populus deltoides×P．euramericana cv ‘Nanlin895’）为材料，通过RACE－PCR技术，获得了NAC1基因的全长cDNA，命名为PdNAC1。该序列含有1个长906 bp的开放阅读框，编码302个氨基酸。该蛋白的分子质量为34．23 ku，等电点为7．44。分析其氨基酸序列发现，该蛋白具有典型NAC转录因子特征，NAM结构域位于11～135个氨基酸之间，含有A、B、C、D、E 5个亚结构域，该区域可以结合DNA；而C端含有一个高度变异的转录激活区。利用杨树原生质体对PdNAC1蛋白进行细胞亚定位，结果发现PdNAC1不仅明显定位于细胞核中，而且在细胞膜上也有表达。但PdNAC1是否具有相应的跨膜结构域尚不明确。
이‘남림895’양（Populus deltoides×P．euramericana cv ‘Nanlin895’）위재료，통과RACE－PCR기술，획득료NAC1기인적전장cDNA，명명위PdNAC1。해서렬함유1개장906 bp적개방열독광，편마302개안기산。해단백적분자질량위34．23 ku，등전점위7．44。분석기안기산서렬발현，해단백구유전형NAC전록인자특정，NAM결구역위우11～135개안기산지간，함유A、B、C、D、E 5개아결구역，해구역가이결합DNA；이C단함유일개고도변이적전록격활구。이용양수원생질체대PdNAC1단백진행세포아정위，결과발현PdNAC1불부명현정위우세포핵중，이차재세포막상야유표체。단PdNAC1시부구유상응적과막결구역상불명학。
NAC transcription factors are the key transcription regulatory factors which involved in plant multiple biologi?cal functions, such as plant development, hormones regulation, and environmental stimuli. The full length cDNA se?quence of a NAC?like gene from the hybrid of Populus deltoides × P. euramericana cv‘Nanlin895’ , was cloned by RT?PCR and RACE methods, named PdNAC1, which contains an ORF of 906 bp and encodes 302 amino acids with the mo?lecular weight of about 34?23 ku ond pI of 7?44. The putative protein sequence analysis showed that PdNAC1contained conserved NAC domain including five sub?domains A, B, C, D, and E which can bind DNA. Meanwhile, the C?termi?nal of PdNAC1 is highly different and that is very typical in NAC transcription factors. Furthermore, the results of sub?cellular localization in poplar protoplast showed that the PdNAC1 transcription factor is mainly located in the cell nucle?us, but also translocated on the cell membrane. However,further investigation would be needed to reveal whether the Pd?NAC1 is corresponding to transmembrane domain.