CAJ | 학술논문

杂交子代的纯度直接影响到遗传图谱的质量，因而在遗传图谱构建前要对杂交子代进行鉴定与选择。笔者从300对SSR标记中，筛选出11对能明确显示亲本来源的标记，利用这11对标记对380株美洲黑杨杂交子代进行了鉴定。结果表明：有372个个体因同时具有亲本各1条等位基因而被认定为亲子代可用于图谱构建，其余8个个体因产生了非亲本来源或额外的等位基因，这些个体可能源于花粉污染或染色体异常，在作图过程中宜从作图群体中排除。
잡교자대적순도직접영향도유전도보적질량，인이재유전도보구건전요대잡교자대진행감정여선택。필자종300대SSR표기중，사선출11대능명학현시친본래원적표기，이용저11대표기대380주미주흑양잡교자대진행료감정。결과표명：유372개개체인동시구유친본각1조등위기인이피인정위친자대가용우도보구건，기여8개개체인산생료비친본래원혹액외적등위기인，저사개체가능원우화분오염혹염색체이상，재작도과정중의종작도군체중배제。
Since the purity of hybrids affects the quality of the genetic map, identification and selection of hybrids should be done before the genetic map construction. In this study, we screened 11 SSR pairs primer that could be used to track the kinship of the hybrids from a total 300 pairs primer. Subsequently, these primers were used to test 380 offsprings from a hybridization of Populus deltoides. Among which, 372 individuals were revealed to own complementary alleles for each parent, and they were identified as true hybrids for genetic map construction. Whereas the remaining eight individu?als, they were observed to own non?parental alleles or extra parental alleles, suggesting these progeny were either resul?ted from pollen contamination or chromosomal abnormalities. Thus they were excluded from map construction.