CAJ | 학술논문

为研究水曲柳合子胚发育后熟时期的基因组表观调控模式，以7月上旬到9月中旬8个取材时期多年生水曲柳合子胚子叶为研究对象，通过基因组甲基化敏感扩增多态性分析，检测了各时期基因组甲基化变化。结果表明：水曲柳合子胚在不同发育时期可检测到933～939条清晰的电泳条带，说明不同发育时期水曲柳合子胚甲基化差异不明显。进一步统计胞嘧啶甲基化模式及其变异模式发现，7月初基因组在CNG、CG／CNG位点下降的变异位点多于其他时期，表明在水曲柳合子胚发育过程中发生了较大规模的去甲基化事件。
위연구수곡류합자배발육후숙시기적기인조표관조공모식，이7월상순도9월중순8개취재시기다년생수곡류합자배자협위연구대상，통과기인조갑기화민감확증다태성분석，검측료각시기기인조갑기화변화。결과표명：수곡류합자배재불동발육시기가검측도933～939조청석적전영조대，설명불동발육시기수곡류합자배갑기화차이불명현。진일보통계포밀정갑기화모식급기변이모식발현，7월초기인조재CNG、CG／CNG위점하강적변이위점다우기타시기，표명재수곡류합자배발육과정중발생료교대규모적거갑기화사건。
To research epigenetic regulation of genome of Fraxinus mandshurica seed development ripening period, Fraxinus mandshurica cotyledons from early July to mid?September 8 stages were selected as test material. Through MSAP ( methylation?sensitive amplified polymorphism) method, we detected the change of DNA ethylation in each peri?od. The result indicated that no significant difference among different development stages was detected ( in total 933-939 clearly identified strips was detected in each stage) . However, the cytosine methylation patterns and variation pattern of CCGG sites showed that, in early July, CNG, CG/CNG loci demethylation sites were higher than other stage, which in?dicated that large scale of DNA demethylation occurred following seed development of Fraxinus mandshurica. This result could serve as the regulation patterns and as an important basis for providing theoretical basis for seed development.