CAJ | 학술논문

为开发碧桃转录组微卫星信息，利用454高通量测序技术，对其花瓣转录组序列进行SSR位点发掘，结果发现含SSR的序列4705条，共得到5668个SSR，平均每3．49 kb出现1个SSR。微卫星序列主要以三碱基重复为主，约占总数的42．66％。笔者共发现516种碱基重复基元，所占比例最高的为（AG／CT）n（18?34％），其次是（ AAG／CTT） n（12?42％）。微卫星多为重复长度小于20 bp 的短序列，长度大于20 bp 的微卫星仅占总数的12?13％。研究还发现碧桃花瓣微卫星的频率和长度呈显著负相关（ P＜0?05），相关系数为－0?246。
위개발벽도전록조미위성신식，이용454고통량측서기술，대기화판전록조서렬진행SSR위점발굴，결과발현함SSR적서렬4705조，공득도5668개SSR，평균매3．49 kb출현1개SSR。미위성서렬주요이삼감기중복위주，약점총수적42．66％。필자공발현516충감기중복기원，소점비례최고적위（AG／CT）n（18?34％），기차시（ AAG／CTT） n（12?42％）。미위성다위중복장도소우20 bp 적단서렬，장도대우20 bp 적미위성부점총수적12?13％。연구환발현벽도화판미위성적빈솔화장도정현저부상관（ P＜0?05），상관계수위－0?246。
To obtain information of Prunus persica cv. duplex, the transcriptome of peach flowers were sequenced by 454 high?throughput sequencing technology. A total of 5 668 SSRs were identified in 4 705 sequences, with an average densi?ty of one SSR per 3.49 kb. Tri?nucleotide repeats were the most abundant, accounted for 42.66% of all SSRs. Among all the 516 SSR motifs, ( AG/CT ) n was the most frequent repeat motif ( 18?34%) , followed by the ( AAG/CTT ) n ( 12?42%) . The microsatellites in length below 20 bp were in maximum proportion, while the microsatellites over 20 bp were only 12.13%. There were significant negative correlation ( P<0.05) between the frequency of microsatellites and the length, the correlation coefficient was -0.246.