CAJ | 학술논문

目的探讨β3肾上腺素受体基因Trp64Arg多态性与我国北方汉族人群肥胖和血脂水平的相关性.方法选取中国北方汉族人群1 602例,其中男995例,女607例,利用TaqMan MGB等位基因分型试剂盒检测患者β3肾上腺素受体基因Trp64Arg多态性分布频率,分析该多态性位点与北方汉族人群体质指数和血脂水平的关系.结果 Trp64Arg位点基因型在本研究人群中的分布符合Hardy-Weinberg平衡(x2=0.043,P=0.087),Arg64等位基因频率为15.82％.在整体人群中,未发现Trp64Arg与体质指数和血脂水平的相关性;以是否合并高血压进行亚组分析,在高血压亚组中,Trp64Trp基因型患者的总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、总胆固醇与高密度脂蛋白胆固醇的比值(TC/HDL)分别为(5.27±1.04) mmol/L、(3.42±0.88) mmol/L、(4.81±1.31),均显著高于Arg64携带者的(5.10±1.02) mmol/L、(3.27±0.87) mmol/L、(4.57±1.25),差异有统计学意义(=2.334、2.067、2.563,均P＜0.05).经多元逐步回归分析,对可能影响高血压患者血脂水平的因素进行校正,结果显示此多态性位点与血脂水平有关,可能影响高血压患者总胆固醇水平3.2％的变异(P =0.030,R2=0.032),影响低密度脂蛋白胆固醇水平2.5％的变异(P =0.030,R2=0.025),可能影响总胆固醇与高密度脂蛋白胆固醇的比值3.4％的变异(P =0.001,R2=0.034).在非高血压组中,未发现Trp64Arg位点与血脂水平的相关性.无论是高血压还是非高血压组中,均未发现该位点与体重指数有关.结论 β3肾上腺素受体基因Trp64Arg多态性与中国北方汉族高血压人群的血脂水平有关,Trp64Trp基因型患者总胆固醇、低密度脂蛋白胆固醇水平更高.
목적 탐토β3신상선소수체기인Trp64Arg다태성여아국북방한족인군비반화혈지수평적상관성.방법 선취중국북방한족인군1 602례,기중남995례,녀607례,이용TaqMan MGB등위기인분형시제합검측환자β3신상선소수체기인Trp64Arg다태성분포빈솔,분석해다태성위점여북방한족인군체질지수화혈지수평적관계.결과 Trp64Arg위점기인형재본연구인군중적분포부합Hardy-Weinberg평형(x2=0.043,P=0.087),Arg64등위기인빈솔위15.82％.재정체인군중,미발현Trp64Arg여체질지수화혈지수평적상관성;이시부합병고혈압진행아조분석,재고혈압아조중,Trp64Trp기인형환자적총담고순(TC)、저밀도지단백담고순(LDL-C)、총담고순여고밀도지단백담고순적비치(TC/HDL)분별위(5.27±1.04) mmol/L、(3.42±0.88) mmol/L、(4.81±1.31),균현저고우Arg64휴대자적(5.10±1.02) mmol/L、(3.27±0.87) mmol/L、(4.57±1.25),차이유통계학의의(=2.334、2.067、2.563,균P＜0.05).경다원축보회귀분석,대가능영향고혈압환자혈지수평적인소진행교정,결과현시차다태성위점여혈지수평유관,가능영향고혈압환자총담고순수평3.2％적변이(P =0.030,R2=0.032),영향저밀도지단백담고순수평2.5％적변이(P =0.030,R2=0.025),가능영향총담고순여고밀도지단백담고순적비치3.4％적변이(P =0.001,R2=0.034).재비고혈압조중,미발현Trp64Arg위점여혈지수평적상관성.무론시고혈압환시비고혈압조중,균미발현해위점여체중지수유관.결론 β3신상선소수체기인Trp64Arg다태성여중국북방한족고혈압인군적혈지수평유관,Trp64Trp기인형환자총담고순、저밀도지단백담고순수평경고.
Objective To investigate the relationship between the Trp64Arg polymorphism of β3-adrenergic receptor (ADRB3) gene and obesity and the levels of blood lipids in the Northern Han Chinese population.Methods A total of 1 602 subjects in northern Han Chinese were recruited in this study,including 995 males and 607 females.Genotyping was performed using the TaqMan assay to identify the Trp64Arg polymorphisms of the ADRB3.The relationship between the polymorphism and obesity and blood lipids was analyzed.Results The genotype distribution for Trp64Arg polymorphism was in conformity with the Hardy-Weinberg equilibrium (HWE) in the recruited population (x2 =0.043,P =0.087).The frequency of Arg64 allele was 15.82％.In the overall analysis,no significant association was showed between the Trp64Arg polymorphism and the Body Mass Index (BMI) or the levels of blood lipids.Subgroup analysis was performed by hypertension.In the hypertensive subgroup,the results showed significant association between the polymorphism and the levels of total cholesterol (TC),low-density lipoprotein cholesterol (LDL-C) and the ratio of TC and high-density lipoprotein cholesterol (TC/HDL-C) (TC:(5.27 ± 1.04) mmol/L vs (5.10 ± 1.02) mmol/L,t =2.334,P =0.02;LDL-C:(3.42 ± 0.88) mmol/L vs (3.27±0.87)mmol/L,t=2.067,P=0.039;TC/HDL:4.81 ±1.31 vs4.57 ±1.25,t=2.563,P=0.011).Stepwise multiple regression analysis showed that there were significant associations between the Trp64Arg polymorphism and the levels of blood lipids.The polymorphism might affect 3.2％ variances of the level of TC (P =0.030,R2 =0.032),2.5％ variances of the level of LDL-C (P =0.030,R2 =0.025),3.4％ variances of the TC/HDL-C ratio (P =0.001,R2 =0.034),respectively.In the normatensive subgroup,there was no significant association between the polymorphism and the levels of lipids.No significant association was observed between the polymorphism and BMI either in the hypertension group or in the normotension group.Conclusions The Trp64Arg polymorphism in ADRB3 gene may be associated with the levels of blood lipids of the Chinese Han patients with essential hypertension.The hypertensive patients with the Trp64Trp genotype may be liable to dyslipidemia.