CAJ | 학술논문

目的探讨糖原合成酶激酶3β(Glycogen synthase kinase-3β,GSK-3β)基因rs334558位点多态性与中国汉族人群重性抑郁障碍(major depressive disorder,MDD)之间的关系.方法以250例MDD患者及300例健康对照作为研究对象,应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术测定研究对象的GSK-3β基因rs334558位点的CC/CT+TT基因型及等位基因频率分布.运用SPSS1Z0进行数据分析.结果 (1)病例组GSK-3β rs334558位点基因分布(CC/CT+TT基因型频率分别为38.4％和61.6％,C、T等位基因频率分别为63.2％和36.8％)与对照组(CC/CT+TT基因型频率分别为43.0％和57.0％,C、T等位基因频率分别为67.2％和32.8％)相比,均差异无统计学意义(x2=1.194,P=0.275;x2=1.895,P=0.169);(2)在女性中,病例组GSK-3β rs334558位点基因分布(CC/CT+TT基因型频率分别为37.9％和62.1％,C、T等位基因频率分别为63.5％和36.5％)与对照组(CC/CT+ TT基因型频率分别为51.0％和49.0％,C、T等位基因频率分别为72.4％和27.6％)相比,均差异有统计学意义(x2=6.499,P=0.011;x2=6.858,P=0.009),女性中携带T等位基因的人群患MDD的危险度是携带C等位基因的1.504倍(OR=1.504,95％ CI=1.107～2.043);(3) GSK-3β基因rs334558位点CC/CT+TT基因型、等位基因与女性MDD抑郁症状的严重程度的关系中均差异无统计学意义(x2=0.519,P=0.471;x2=0.199,P=0.656).结论 GSK-3β基因rs334558位点多态性可能与中国女性人群MDD发病有关,且携带T等位基因可能增加女性患MDD的危险性,但该基因位点多态性对女性MDD的抑郁严重程度无影响.
목적 탐토당원합성매격매3β(Glycogen synthase kinase-3β,GSK-3β)기인rs334558위점다태성여중국한족인군중성억욱장애(major depressive disorder,MDD)지간적관계.방법 이250례MDD환자급300례건강대조작위연구대상,응용취합매련반응-한제성편단장도다태성(PCR-RFLP)기술측정연구대상적GSK-3β기인rs334558위점적CC/CT+TT기인형급등위기인빈솔분포.운용SPSS1Z0진행수거분석.결과 (1)병례조GSK-3β rs334558위점기인분포(CC/CT+TT기인형빈솔분별위38.4％화61.6％,C、T등위기인빈솔분별위63.2％화36.8％)여대조조(CC/CT+TT기인형빈솔분별위43.0％화57.0％,C、T등위기인빈솔분별위67.2％화32.8％)상비,균차이무통계학의의(x2=1.194,P=0.275;x2=1.895,P=0.169);(2)재녀성중,병례조GSK-3β rs334558위점기인분포(CC/CT+TT기인형빈솔분별위37.9％화62.1％,C、T등위기인빈솔분별위63.5％화36.5％)여대조조(CC/CT+ TT기인형빈솔분별위51.0％화49.0％,C、T등위기인빈솔분별위72.4％화27.6％)상비,균차이유통계학의의(x2=6.499,P=0.011;x2=6.858,P=0.009),녀성중휴대T등위기인적인군환MDD적위험도시휴대C등위기인적1.504배(OR=1.504,95％ CI=1.107～2.043);(3) GSK-3β기인rs334558위점CC/CT+TT기인형、등위기인여녀성MDD억욱증상적엄중정도적관계중균차이무통계학의의(x2=0.519,P=0.471;x2=0.199,P=0.656).결론 GSK-3β기인rs334558위점다태성가능여중국녀성인군MDD발병유관,차휴대T등위기인가능증가녀성환MDD적위험성,단해기인위점다태성대녀성MDD적억욱엄중정도무영향.
Objective To explore the association between glycogen synthase kinase-3β (GSK-3β) rs334558 gene polymorphism and major depressive disorder (MDD) in Han Chinese.Methods Genotypes and alleles frequency of GSK-3β rs334558 polymorphism were examined in 250 patients and 300 healthy controls by the method of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).SPSS 17.0 statistical software was used for statistical analysis.Results (1) GSK-3β rs334558 locus of CC,CT+TT genotypes and alleles frequency distribution between patient group (CC,CT+TT genotypes:38.4％,61.6％.C,T alleles:63.2％,36.8％) and control group (CC,CT+TT genotypes:43.0％,57.0％.C,T alleles:67.2％,32.8％) had no significant difference (x2 =1.194,P=0.275.x2 =1.895 P=0.169);(2) There were significant differences of rs334558 genotypes and alleles between patient group (CC,CT+TT genotypes:37.9％,62.1％.C,T alleles:63.5％,36.5％) and control group (CC,CT+TT genotypes:51.0％,49.0％.C,T alleles:72.4％,27.6％) in female (x2=6.499,P =0.011.x2 =6.858,P=0.009).T allele was a risk factor for female MDD compared with C allele (OR=1.504,95％ CI =1.107 ～ 2.043);(3) There was no association between rs334558 polymorphism and MDD severity in female (x2=0.519,P=0.471.x2=0.199,P=0.656).Conclusion This study suggests that GSK-3β rs334558 polymorphism may be associated with the onset of MDD among female,and T allele may be a risk factor.But there is no association between polymorphism and severity of female MDD.