CAJ | 학술논문

目的研究载脂蛋白E基因(apolipoprotein E gene,APOE)多态性与老年期抑郁症(geriatricdepression,GD)的关联,并探讨APOE基因型与患者抑郁症状、血脂水平及认知功能之间的关系.方法采用单核苷酸多态性(single nucleotide polymorphism,SNP)位点检测技术检测120例GD患者及120例健康对照者APOE的rs7412及rs429358两多态性位点,分出各基因型;分析两组各基因型及等位基因频率差异;分析病例组APOE多态性与患者临床及人口学资料间的关系;采用Logistic多元回归分析各一般资料、临床资料对GD认知功能的影响.结果两组基因型及各等位基因频率均差异无统计学意义(均P＞0.05);携带ε4基因型的患者简易智能量表总分低于非携带者[(22.38±2.22)分,(25.28±2.28)分,t=3.091,P＜0.01],而血脂水平TC(t=2.225,P＜0.05)、LDL(t=2.728,P＜0.01)高于非携带者;携带e4患者典型临床表现为阻滞(载荷0.695)和认知障碍(载荷0.902),而非携带者突出表现在焦虑躯体化因子(载荷0.990)和体质量因子(载荷0.864);GD认知损害的危险因子按作用大小依次为携带ε4的基因型(b '=1.097)、TC(b'=0.401).结论 APOE可能并非GD的发病因子;携带ε4基因型的患者认知功能差,血脂水平高;不同APOE基因型患者有不同的临床表现.
목적 연구재지단백E기인(apolipoprotein E gene,APOE)다태성여노년기억욱증(geriatricdepression,GD)적관련,병탐토APOE기인형여환자억욱증상、혈지수평급인지공능지간적관계.방법 채용단핵감산다태성(single nucleotide polymorphism,SNP)위점검측기술검측120례GD환자급120례건강대조자APOE적rs7412급rs429358량다태성위점,분출각기인형;분석량조각기인형급등위기인빈솔차이;분석병례조APOE다태성여환자림상급인구학자료간적관계;채용Logistic다원회귀분석각일반자료、림상자료대GD인지공능적영향.결과 량조기인형급각등위기인빈솔균차이무통계학의의(균P＞0.05);휴대ε4기인형적환자간역지능량표총분저우비휴대자[(22.38±2.22)분,(25.28±2.28)분,t=3.091,P＜0.01],이혈지수평TC(t=2.225,P＜0.05)、LDL(t=2.728,P＜0.01)고우비휴대자;휴대e4환자전형림상표현위조체(재하0.695)화인지장애(재하0.902),이비휴대자돌출표현재초필구체화인자(재하0.990)화체질량인자(재하0.864);GD인지손해적위험인자안작용대소의차위휴대ε4적기인형(b '=1.097)、TC(b'=0.401).결론 APOE가능병비GD적발병인자;휴대ε4기인형적환자인지공능차,혈지수평고;불동APOE기인형환자유불동적림상표현.
Objective To examine the association between the polymorphisms of apolipoprotein E gene (APOE) and geriatric depression (GD),and then conduct an exploratory investigation to analyse whether the APOE polymorphisms would relate to the depressive syndrome severity,the cognitive function,or the level of serum lipid in patients.Methods Participants,including 120 GD patients and 120 normal controls were enrolled to detect the two single nucleotide polymorphisms of APOE,rs7412 and rs429358 using the technology of SNP site testing.The frequency differences of genotype and allele were compared between the two groups.Then the association between APOE polymorphisms and clinical or demographic data of patients were clarified.The relationship between clinical or demographic data,and the cognitive function of GD patients were investigated using the Logistic multiple regression analysis.Results The frequency of APOE genotype and allele showed no significant difference between the two groups(P＞0.05).Patients carrying e4 allen had significantly lower total scores of Man-Machine System Engineering((22.38±2.22) vs (25.28±2.28),t=3.091,P＜0.01) and higher levels of TC (t=2.225,P＜ 0.05) and LDL(t=2.728,P＜0.01) compared with those without ε4 allen.The specific symptoms of patients carrying e4 allen were cognitive impairment(load 0.902) and retardant factors(load 0.695),while patients without ε4 allen had characteristic symptoms of anxiety(load 0.990) and weight factors(load 0.864).Ranked by the effect power,the risk factors of cognitive impairment of GD patients are ε4(b'=1.097) and then TCTC (b'=0.401).Conclusions APOE may not modulate the susceptibility to GD.Patients carrying ε4 allen have severer cognitive impairment and higher levels of serum lipid.The different genotypes may lead to different clinical symptoms.