中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2015年
20期
1599-1601
,共3页
唐一君%瞿文%王一浩%刘惠%丁少雪%张洋%李丽娟%付蓉%王化泉
唐一君%瞿文%王一浩%劉惠%丁少雪%張洋%李麗娟%付蓉%王化泉
당일군%구문%왕일호%류혜%정소설%장양%리려연%부용%왕화천
血小板减少%白细胞介素10%调节性B细胞
血小闆減少%白細胞介素10%調節性B細胞
혈소판감소%백세포개소10%조절성B세포
Thrombocytopenia%Interleukin-10%Regulatory B cells
目的 探讨免疫性血小板减少症(ITP)患者外周血中调节性B细胞(Breg)数量及功能变化.方法 选取2013年11月至2014年10月天津医科大学总医院血液科住院的初诊ITP患者35例、完全缓解ITP患者32例及健康志愿者30名.采用流式细胞术检测其外周血Breg数量(CD19+ CD24hiCD38hi/CD19+)及其功能分子白细胞介素10(IL-10)表达量,并将Breg与ITP患者的免疫状态及血小板计数进行相关性分析.结果 外周血Breg占CD19+淋巴细胞比例:ITP初诊患者(3.09％±2.57％)显著低于完全缓解患者(7.78％±6.59％,P =0.002)和健康对照(5.42％±3.31％,P=0.003).外周血Breg中IL-10+细胞比例:初诊患者比例(32.30％±12.71％)低于完全缓解患者(71.73％±18.08％,P=0.000)和健康对照(51.01％ ±17.08％,P=0.026),完全缓解患者高于健康对照(P =0.026).Breg与髓样树突细胞(mDC)数量呈正相关(r=0.644,p=0.003),与外周血血小板计数呈正相关(r=0.327,P=0.006).结论 Breg参与了ITP的发病,是其潜在的治疗靶点.
目的 探討免疫性血小闆減少癥(ITP)患者外週血中調節性B細胞(Breg)數量及功能變化.方法 選取2013年11月至2014年10月天津醫科大學總醫院血液科住院的初診ITP患者35例、完全緩解ITP患者32例及健康誌願者30名.採用流式細胞術檢測其外週血Breg數量(CD19+ CD24hiCD38hi/CD19+)及其功能分子白細胞介素10(IL-10)錶達量,併將Breg與ITP患者的免疫狀態及血小闆計數進行相關性分析.結果 外週血Breg佔CD19+淋巴細胞比例:ITP初診患者(3.09％±2.57％)顯著低于完全緩解患者(7.78％±6.59％,P =0.002)和健康對照(5.42％±3.31％,P=0.003).外週血Breg中IL-10+細胞比例:初診患者比例(32.30％±12.71％)低于完全緩解患者(71.73％±18.08％,P=0.000)和健康對照(51.01％ ±17.08％,P=0.026),完全緩解患者高于健康對照(P =0.026).Breg與髓樣樹突細胞(mDC)數量呈正相關(r=0.644,p=0.003),與外週血血小闆計數呈正相關(r=0.327,P=0.006).結論 Breg參與瞭ITP的髮病,是其潛在的治療靶點.
목적 탐토면역성혈소판감소증(ITP)환자외주혈중조절성B세포(Breg)수량급공능변화.방법 선취2013년11월지2014년10월천진의과대학총의원혈액과주원적초진ITP환자35례、완전완해ITP환자32례급건강지원자30명.채용류식세포술검측기외주혈Breg수량(CD19+ CD24hiCD38hi/CD19+)급기공능분자백세포개소10(IL-10)표체량,병장Breg여ITP환자적면역상태급혈소판계수진행상관성분석.결과 외주혈Breg점CD19+림파세포비례:ITP초진환자(3.09％±2.57％)현저저우완전완해환자(7.78％±6.59％,P =0.002)화건강대조(5.42％±3.31％,P=0.003).외주혈Breg중IL-10+세포비례:초진환자비례(32.30％±12.71％)저우완전완해환자(71.73％±18.08％,P=0.000)화건강대조(51.01％ ±17.08％,P=0.026),완전완해환자고우건강대조(P =0.026).Breg여수양수돌세포(mDC)수량정정상관(r=0.644,p=0.003),여외주혈혈소판계수정정상관(r=0.327,P=0.006).결론 Breg삼여료ITP적발병,시기잠재적치료파점.
Objective To explore the quantity and function of regulatory B cells (Bregs) in peripheral blood (PB)of the patients with immune thrombocytopenia(ITP).Methods A total of 67 ITP patients (35 patients were newly diagnosed,32 patients were in remission) were collected from November 2013 to October 2014 in our hospital.And 30 normal controls were enrolled in this study.Their PB Bregs (CD19 + CD24hiCD38hicells) and related molecules interleukin-10(IL-10) in Bregs were detected by flow cytometry (FCM).Correlation between Bregs and clinical parameters of ITP patients was analysed.Results The percentage of Bregs in CD19 + cells of newly diagnosed ITP patients(3.09％ ± 2.57％)was significantly lower than that of remitted ITP patients (7.78％ ± 6.59％,P =0.002) and controls (5.42％ ± 3.31％,P =0.003).The percentage of IL-10 + cells in Bregs of newly diagnosed ITP patients (32.30％ ± 12.71％) was significantly lower than that of remitted ITP patients (71.73％ ± 18.08％,P =0.000) and controls (51.01％ ± 17.08％,P =0.026).And the percentage of IL-10 + cells in Bregs of remitted ITP patients was significantly higher than that of controls (P =0.026).The level of Bregs of newly diagnosed ITP patients had significantly positive correlation with myeloid dendritic cells (mDC) (r =0.644,P =0.003) and PB platelet count(r =0.327,P =0.006).Conclusion Bregs involved in the pathogenesis of ITP might be a potential therapeutic target of this disease.
