中国医药
中國醫藥
중국의약
CHINA MEDICINE
2015年
6期
865-868
,共4页
白头翁汤%结直肠癌%基因表达谱%基因芯片
白頭翁湯%結直腸癌%基因錶達譜%基因芯片
백두옹탕%결직장암%기인표체보%기인심편
Baitouweng decoction%Colorectal cancer%Gene expression profile%Gene chip
目的 研究白头翁汤治疗对湿热内蕴证结直肠癌患者基因表达谱的影响.方法 收集经病理证实的结肠癌患者5例,辨证均属湿热内蕴.白头翁汤治疗21 d,于治疗前及治疗后取血抽提、纯化mRNA,采用Agilent表达谱芯片进行杂交,计算机软件筛选差异表达基因.结果 与治疗前比较,共筛选出159个差异表达基因,其中表达上调38个,下调121个,差异表达最多的基因为与蛋白结合相关的基因,余者包括与代谢信号转导及物质运输等作用有关的基因.结论 白头翁汤可影响湿热内蕴证结直肠癌患者细胞内一系列基因的表达,这些基因可能与其抗肿瘤机制有关.
目的 研究白頭翁湯治療對濕熱內蘊證結直腸癌患者基因錶達譜的影響.方法 收集經病理證實的結腸癌患者5例,辨證均屬濕熱內蘊.白頭翁湯治療21 d,于治療前及治療後取血抽提、純化mRNA,採用Agilent錶達譜芯片進行雜交,計算機軟件篩選差異錶達基因.結果 與治療前比較,共篩選齣159箇差異錶達基因,其中錶達上調38箇,下調121箇,差異錶達最多的基因為與蛋白結閤相關的基因,餘者包括與代謝信號轉導及物質運輸等作用有關的基因.結論 白頭翁湯可影響濕熱內蘊證結直腸癌患者細胞內一繫列基因的錶達,這些基因可能與其抗腫瘤機製有關.
목적 연구백두옹탕치료대습열내온증결직장암환자기인표체보적영향.방법 수집경병리증실적결장암환자5례,변증균속습열내온.백두옹탕치료21 d,우치료전급치료후취혈추제、순화mRNA,채용Agilent표체보심편진행잡교,계산궤연건사선차이표체기인.결과 여치료전비교,공사선출159개차이표체기인,기중표체상조38개,하조121개,차이표체최다적기인위여단백결합상관적기인,여자포괄여대사신호전도급물질운수등작용유관적기인.결론 백두옹탕가영향습열내온증결직장암환자세포내일계렬기인적표체,저사기인가능여기항종류궤제유관.
Objective To investigate the effect of Baitouweng decoction on gene expression profile in damp-heat colorectal cancer using gene chip technology.Methods Five damp-heat type patients with colorectal cancer confirmed by pathology were administrated with Baitouweng decoction for 21 days.Before and after treatment,the blood was taken and the total mRNA was extracted and purified.Agilent gene expression profile chip was used to hybridize and the differentially expressed genes were screened.Results Compared with pretreatment,159 differentially expressed genes were found,including 38 genes up-regulated and 121 genes down-regulated.Most of the differentially expressed genes were related with protein binding;others were related with metabolism,signal conduction and material transportation.Conclusion In treatment of damp-heat colorectal cancer patients,Baitouweng decoction influences the expression of a series of genes,which are related to antitumor effects.
