中华老年医学杂志
中華老年醫學雜誌
중화노년의학잡지
Chinese Journal of Geriatrics
2015年
5期
553-556
,共4页
胡哲夫%唐其柱%刘源%李金%张文斌
鬍哲伕%唐其柱%劉源%李金%張文斌
호철부%당기주%류원%리금%장문빈
番茄%血管紧张素Ⅱ%肌细胞,心脏%细胞凋亡
番茄%血管緊張素Ⅱ%肌細胞,心髒%細胞凋亡
번가%혈관긴장소Ⅱ%기세포,심장%세포조망
Lycopersicon esculentum%Angiotensin Ⅱ%Myocytes,cardiac%Apoptosis
目的 研究番茄红素(Lyc)对血管紧张素Ⅱ(AngⅡ)诱导H9c2心肌细胞凋亡的保护作用,并探讨其可能的机制.方法 采用AngⅡ(10 μmol/L)刺激H9c2细胞,观察不同浓度Lyc对AngⅡ诱导细胞凋亡的保护效果.采用CCK8检测不同浓度Lyc和(或)AngⅡ对H9c2细胞活性的影响;采用实时半定量逆转录聚合酶链反应(RT-PCR)检测细胞中Bax、Bcl-2的基因表达水平;免疫印迹(Western blot)法检测细胞中Bax、Caspase3、Caspase9、Bcl-2的蛋白表达量;TUNEL染色观察细胞的凋亡比率.结果 和对照组相比,AngⅡ组中H9c2细胞活性为(92.87±4.37)％,当Lyc与AngⅡ同时干预H9c2细胞后,细胞活性显著上升(P＜0.05);RT-PCR结果显示AngⅡ可上调H9c2胞内Bax mRNA表达,Bcl-2表达下调;当AngⅡ与Lyc同时作用时,Bax表达下调而Bcl-2表达上调,且这种改变具有浓度依赖性;Bax、Caspase3、Caspase9在AngⅡ的作用下蛋白表达量上调,Bcl-2下调(P＜0.05),而AngⅡ与Lyc同时作用时可逆转这一现象.TUNEL染色结果显示,AngⅡ作用于H9c2细胞时,可见大量凋亡细胞;而Lyc与AngⅡ同时作用时,凋亡细胞的数量明显减少(P＜0.05).结论 Lyc能够明显改善AngⅡ诱导的H9c2心肌细胞凋亡,提示其对于改善临床上各种心血管疾病可能有着重要的作用.
目的 研究番茄紅素(Lyc)對血管緊張素Ⅱ(AngⅡ)誘導H9c2心肌細胞凋亡的保護作用,併探討其可能的機製.方法 採用AngⅡ(10 μmol/L)刺激H9c2細胞,觀察不同濃度Lyc對AngⅡ誘導細胞凋亡的保護效果.採用CCK8檢測不同濃度Lyc和(或)AngⅡ對H9c2細胞活性的影響;採用實時半定量逆轉錄聚閤酶鏈反應(RT-PCR)檢測細胞中Bax、Bcl-2的基因錶達水平;免疫印跡(Western blot)法檢測細胞中Bax、Caspase3、Caspase9、Bcl-2的蛋白錶達量;TUNEL染色觀察細胞的凋亡比率.結果 和對照組相比,AngⅡ組中H9c2細胞活性為(92.87±4.37)％,噹Lyc與AngⅡ同時榦預H9c2細胞後,細胞活性顯著上升(P＜0.05);RT-PCR結果顯示AngⅡ可上調H9c2胞內Bax mRNA錶達,Bcl-2錶達下調;噹AngⅡ與Lyc同時作用時,Bax錶達下調而Bcl-2錶達上調,且這種改變具有濃度依賴性;Bax、Caspase3、Caspase9在AngⅡ的作用下蛋白錶達量上調,Bcl-2下調(P＜0.05),而AngⅡ與Lyc同時作用時可逆轉這一現象.TUNEL染色結果顯示,AngⅡ作用于H9c2細胞時,可見大量凋亡細胞;而Lyc與AngⅡ同時作用時,凋亡細胞的數量明顯減少(P＜0.05).結論 Lyc能夠明顯改善AngⅡ誘導的H9c2心肌細胞凋亡,提示其對于改善臨床上各種心血管疾病可能有著重要的作用.
목적 연구번가홍소(Lyc)대혈관긴장소Ⅱ(AngⅡ)유도H9c2심기세포조망적보호작용,병탐토기가능적궤제.방법 채용AngⅡ(10 μmol/L)자격H9c2세포,관찰불동농도Lyc대AngⅡ유도세포조망적보호효과.채용CCK8검측불동농도Lyc화(혹)AngⅡ대H9c2세포활성적영향;채용실시반정량역전록취합매련반응(RT-PCR)검측세포중Bax、Bcl-2적기인표체수평;면역인적(Western blot)법검측세포중Bax、Caspase3、Caspase9、Bcl-2적단백표체량;TUNEL염색관찰세포적조망비솔.결과 화대조조상비,AngⅡ조중H9c2세포활성위(92.87±4.37)％,당Lyc여AngⅡ동시간예H9c2세포후,세포활성현저상승(P＜0.05);RT-PCR결과현시AngⅡ가상조H9c2포내Bax mRNA표체,Bcl-2표체하조;당AngⅡ여Lyc동시작용시,Bax표체하조이Bcl-2표체상조,차저충개변구유농도의뢰성;Bax、Caspase3、Caspase9재AngⅡ적작용하단백표체량상조,Bcl-2하조(P＜0.05),이AngⅡ여Lyc동시작용시가역전저일현상.TUNEL염색결과현시,AngⅡ작용우H9c2세포시,가견대량조망세포;이Lyc여AngⅡ동시작용시,조망세포적수량명현감소(P＜0.05).결론 Lyc능구명현개선AngⅡ유도적H9c2심기세포조망,제시기대우개선림상상각충심혈관질병가능유착중요적작용.
Objective To investigate the effect of lycopene (Lyc) on H9c2 cell apoptosis induced by angiotensin Ⅱ (Ang Ⅱ).Methods Using Ang Ⅱ (10 μmol/L) to stimulate H9c2 cells,we observed the protective effect of Lyc on H9c2 cells apoptosis.The H9c2 cells viability induced by different consideration of Lyc or Ang Ⅱ or both was detected by CCK8 assay.The expression levels of Bax and Bcl-2 in H9c2 cells were determined by real-time quantitative reverse transcription polymerase chain reaction (RT-PCR).Western blot was conducted to detect the protein expressions of Bax,Caspase 3,Caspase 9 and Bcl-2 in H9c2 cells.The apoptotic ratio of H9c2 cells was observed by TUNEL assay.Results Compared with control group,Ang Ⅱ could decrease the viability of H9c2 cells to (92.87±4.37)％.The result of RT-PCR showed that Ang Ⅱ decreased the expression level of Bcl-2,and Bax level was increased under the stimulation of Ang Ⅱ (P＜0.05),while the expression level of Bcl-2 was increased and Bax level was decreased under the co-stimulation of Ang Ⅱ and Lyc in a concentration dependent manner,which indicated that Lyc ameliorated the apoptosis of H9c2 cells.The result of western blot showed that the protein expressions of Bax,Caspase 3 and Caspase 9 were increased,but Bcl-2 was decreased after the stimulation of Ang Ⅱ (P＜0.05).While these phenomenon reversed apparently under the co stimulation of Ang Ⅱ and Lyc.A large number of apoptotic cells were observed under the stimulation of Ang Ⅱ through TUNEL assay.But the number of apoptotic cells reduced significantly under the co-stimulation of Lyc and Ang Ⅱ (P ＜0.05).Conclusions Lyc ameliorates the H9c2 cell apoptosis induced by Ang Ⅱ,which indicates that Lyc may have an important role in the treatment of various cardiovascular diseases.
