CAJ | 학술논문

目的探讨LIG4和HSPB1基因单核苷酸多态性与鼻咽癌预后的关系.方法采用Taqman real-time PCR对168例鼻咽癌患者外周血DNA进行单核苷酸多态性的基因分型.以单因素和多因素Logistic回归计算比值比(OR)及95％可信区间(CI)表示相对危险度;单变量分析用Long-rank检验,多变量分析用COX比例风险模型.结果单因素分析及纳入年龄、性别、吸烟状态、饮酒、病理类型及临床分期多因素分析显示LIG4 C/T(rs1805388)和HSPB1 C/G(rs2868371)位点多态性与鼻咽癌复发、转移及总生存无关.结论 LIG4 rs1805388和HSPB1 rs2868371基因多态性与鼻咽癌预后无关.
목적 탐토LIG4화HSPB1기인단핵감산다태성여비인암예후적관계.방법 채용Taqman real-time PCR대168례비인암환자외주혈DNA진행단핵감산다태성적기인분형.이단인소화다인소Logistic회귀계산비치비(OR)급95％가신구간(CI)표시상대위험도;단변량분석용Long-rank검험,다변량분석용COX비례풍험모형.결과 단인소분석급납입년령、성별、흡연상태、음주、병리류형급림상분기다인소분석현시LIG4 C/T(rs1805388)화HSPB1 C/G(rs2868371)위점다태성여비인암복발、전이급총생존무관.결론 LIG4 rs1805388화HSPB1 rs2868371기인다태성여비인암예후무관.
Objective To investigate the relationship between genetic single-nucleotide polymorphisms of ligase Ⅳ (LIG4) and heat shock protein B1 (HSPB1) and prognosis in nasopharyngeal carcinomas.Methods DNAs were extracted by phenol-chlorofrom method from peripheral blood samples of 168 nasopharyngeal carcinoma patients.Genetic single-nucleotide polymorphisms were divided by Taqman realtime polymerase chain reaction (PCR).All statistical analyses were performed with statistical product and service solutions v13.0.A p-value of less than 0.05 was confirmed as statistical significance.Single-factor and multiple-factor logistic analyses were used to calculate odds ratio (OR) and 95％ confidence interval (CI).Long-rank test and COX were also used.Results No relationship was found between the recurrence,metastasis and overall survival of nasopharyngeal carcinoma and genetic single-nucleotide polymorphisms of LIG4 and HSPB1 with single-factor and multiple-factor logistic analyses.Conclusions LIG4 rs1805388 and HSPB1 rs2868371 had no relationship with prognosis of nasopharyngeal carcinomas.