CAJ | 학술논문

目的评价臭氧(O3)对腰椎间盘突出症患者髓核NO和IL-6水平的影响.方法经椎间孔镜取出16例腰椎间盘突出症患者的髓核,每个髓核均分为3份,采用随机数字表法,将其分为3组(n=16):对照组(C组)不施加任何干预;O320μg/ml组(O320组)和O340μg/ml组(O340组)分别注入20 μg/ml O3和40 μg/ml O3各2 ml.20 min后体外培养72 h,收集培养液,采用硝酸酶还原法检测NO浓度,采用ELISA法检测IL-6浓度;采用实时荧光定量PCR法检测诱导型一氧化氮合酶(iNOS) mRNA和IL-6 mRNA的表达水平.结果与C组比较,O320组和O340组髓核培养液NO和IL-6的浓度降低,髓核组织iNOS mRNA和IL-6 mRNA的表达下调(P＜0.05);O320组和O340组间上述指标比较差异无统计学意义(P＞ 0.05).结论 O3治疗腰椎间盘突出症患者腰腿痛的机制与其减少椎间盘NO合成和降低局部炎性反应有关.
목적 평개취양(O3)대요추간반돌출증환자수핵NO화IL-6수평적영향.방법 경추간공경취출16례요추간반돌출증환자적수핵,매개수핵균분위3빈,채용수궤수자표법,장기분위3조(n=16):대조조(C조)불시가임하간예;O320μg/ml조(O320조)화O340μg/ml조(O340조)분별주입20 μg/ml O3화40 μg/ml O3각2 ml.20 min후체외배양72 h,수집배양액,채용초산매환원법검측NO농도,채용ELISA법검측IL-6농도;채용실시형광정량PCR법검측유도형일양화담합매(iNOS) mRNA화IL-6 mRNA적표체수평.결과 여C조비교,O320조화O340조수핵배양액NO화IL-6적농도강저,수핵조직iNOS mRNA화IL-6 mRNA적표체하조(P＜0.05);O320조화O340조간상술지표비교차이무통계학의의(P＞ 0.05).결론 O3치료요추간반돌출증환자요퇴통적궤제여기감소추간반NO합성화강저국부염성반응유관.
Objective To evaluate the effects of ozone (O3) on the expression of nitric oxide (NO) and interleukin-6 (IL-6) in the nucleus pulposus of patients with lumbar intervertebral discherniation in vitro.Methods Nucleus pulposus specimens were obtained from 16 patients with lumbar intervertebral disc herniation under intervertebral foramen endoscope.Each specimen was randomly divided into 3 groups (n=16 each) using a random number table:control group (group C),O320 μg/ml group (O320 group) and O340 μg/ml group (O340 group).O320 and 40 μg/ml 2 ml were injected into the nucleus pulposus in O320 and O340 groups,respectively.The nucleus pulposus was cultured for 72 h in vitro 20 min later.The culture medium was collected for detection of NO and IL-6 concentrations.The nucleus pulposus tissues were obtained for determination of the expression of inducible nitric oxide synthase (iNOS) and IL-6 mRNA using real-time fluorescent quantitative PCR.Results Compared with group C,the concentrations of NO and IL-6 in the culture medium were significantly decreased,and the expression of iNOS and IL-6 mRNA in nucleus pulposus tissues was down-regulated in O320 and O340 groups.There were no significant differences in the parameters mentioned above between O320 group and O340 group.Conclusion The mechanism by which O3 treats pain in waist and legs is related to reduced production of NO in the intervetebral disc and decreased local inflammatory responses in the patients with lumbar intervertebral disc herniation.