CAJ | 학술논문

目的探讨miR-155在初诊急性髓系白血病(AML)患儿的骨髓单个核细胞中的表达情况,并分析其意义.方法从标本库抽取52例初诊AML患儿骨髓标本,进行总RNA抽提及miR-155特定的cDNA反转录,以30例非恶性病儿童骨髓作为对照.按照国家综合癌症网(NCCN)2013 AML预后分组标准分为预后良好组、中等组和不良组.利用实时荧光定量聚合酶链反应(qRT-PCR)法检测miR-155的表达水平(以2-△△Ct表示).结果 miR-155在AML组显著高于对照组(Z=-5.391,P＜0.001);miR-155在预后良好、中等和不良组之间差异有统计学意义(x2=12.586,P=0.002),预后良好组呈低表达;在不同突变类型之间miR-155的表达水平差异亦有统计学意义(x2=11.216,P=0.024),以FLT3-TKD基因突变组最低,FLT3-ITD基因突变组最高;在不同融合基因分组间差异有统计学意义(x2=12.254,P=0.016),AML-ETO组最高,PML-RARa组最低;按法-美-英(FAB)分型后,各亚型间的miR-155表达水平差异有统计学意义(x2=17.814,P=0.013),M3组较非M3组明显降低(Z=-3.291,P=0.001).结论 miR-155在初诊儿童AML中呈现非常显著的高表达现象,低水平的miR-155与良好预后相关.
목적 탐토miR-155재초진급성수계백혈병(AML)환인적골수단개핵세포중적표체정황,병분석기의의.방법 종표본고추취52례초진AML환인골수표본,진행총RNA추제급miR-155특정적cDNA반전록,이30례비악성병인동골수작위대조.안조국가종합암증망(NCCN)2013 AML예후분조표준분위예후량호조、중등조화불량조.이용실시형광정량취합매련반응(qRT-PCR)법검측miR-155적표체수평(이2-△△Ct표시).결과 miR-155재AML조현저고우대조조(Z=-5.391,P＜0.001);miR-155재예후량호、중등화불량조지간차이유통계학의의(x2=12.586,P=0.002),예후량호조정저표체;재불동돌변류형지간miR-155적표체수평차이역유통계학의의(x2=11.216,P=0.024),이FLT3-TKD기인돌변조최저,FLT3-ITD기인돌변조최고;재불동융합기인분조간차이유통계학의의(x2=12.254,P=0.016),AML-ETO조최고,PML-RARa조최저;안법-미-영(FAB)분형후,각아형간적miR-155표체수평차이유통계학의의(x2=17.814,P=0.013),M3조교비M3조명현강저(Z=-3.291,P=0.001).결론 miR-155재초진인동AML중정현비상현저적고표체현상,저수평적miR-155여량호예후상관.
Objective To investigate the differential expression of miR-155 in newly diagnosed pediatric acute myeloid leukemia(AML) and its clinical significances.Methods Fifty-two AML children and 30 non-malignant disease matched children were recruited as the controls.The preliminary AML children were divided into favorable group,moderate group and poor group according to the National Comprehensive Cancer Network(NCCN) 2013.Real-time quantitative polymerase chain reaction was applied to validate the expressions of miR-155 in bone marrow samples (the data presented by 2-△△Ct).Results By comparing expressions of miR-155 between AML patients and controls,the miR-155 expressions were significantly higher in the AML children than those in the controls (Z =-5.391,P ＜ 0.001).There were significant differences among different prognostic groups,with a significantly lower level in the favorable group compared with others (x2 =12.586,P =0.002).It was also found that differential expressions existed not only in kinds of mutation cohort,with the highest level in FLT3-ITD and the lowest one in FLT3-TKD mutation group (x2 =11.216,P =0.024),but also among fusion gene subgroups (x2 =12.254,P =0.016),with the highest level in AML-ETO group and the lowest level in PML-RARa group:meanwhile,the expressions of miR-155 were statistic different according to French-America-British (FAB) subtypes (x2 =17.814,P =0.013),which was lower in M3 patients than non-M3 patients (Z =-3.291,P =0.001).Conclusions It indicates that the expressions of miR-155 may increase sharply in preliminary AML children,and the lower expression of miR-155 is closely related to favorable prognosis.