中华实用儿科临床杂志
中華實用兒科臨床雜誌
중화실용인과림상잡지
Journal of Applied Clinical Pediatrics
2015年
9期
698-701
,共4页
朱丽华%施燕%王诗雨%郭晓菁%刘慧娟%蒋犁
硃麗華%施燕%王詩雨%郭曉菁%劉慧娟%蔣犛
주려화%시연%왕시우%곽효정%류혜연%장리
促红细胞生成素%脑室周围白质损伤%缺氧缺血%未成熟鼠,新生
促紅細胞生成素%腦室週圍白質損傷%缺氧缺血%未成熟鼠,新生
촉홍세포생성소%뇌실주위백질손상%결양결혈%미성숙서,신생
Erythropoietin%Periventricular white matter damage%Hypoxia-ischemia%Immature rat,new born
目的 探讨人重组促红细胞生成素(rh-EPO)在早产鼠脑室周围白质损伤模型鼠脑白质中的促血管新生作用.方法 选取出生3日龄SD大鼠,随机数字表法分为假手术(Sham)组,缺氧缺血(HI)组和EPO治疗组.HI组和EPO治疗组幼鼠结扎右侧颈总动脉并吸入60 mL/L的氮氧混合气体4h,Sham组幼鼠仅游离右侧颈总动脉.EPO治疗组术后即经腹腔给予大剂量rh-EPO(5 U/g)1次,Sham组和HI组给予等量的9 g/L盐水.采用免疫组织化学、Western blot和实时荧光定量PCR(qRT-PCR)方法检测术后48 h及96 h大鼠脑白质中微血管密度(MVD)、CD34蛋白、动静脉标志物(ephrinB2和EphB4)表达情况.结果 术后48 h,HI组CD34蛋白(0.54±0.05)较Sham组(0.42±0.05)增加(P＜0.05),但HI组MVD、ephrinB2和EphB4与Sham组比较表达未见明显改变(P ＞0.05);EPO治疗组CD34蛋白(0.74±0.07)、ephrinB2(2.07±0.58)和EphB4 (2.13±0.44)表达则较HI组(CD34蛋白:0.54±0.05,ephrinB2:0.70±0.23,EphB4:0.67±0.07)均明显增加(P均＜0.05),但是MVD未见明显增多(P＞0.05).术后96 h,HI组CD34蛋白(0.85±0.06)、MVD(3.14±1.21)、ephrinB2(7.51 ±1.89)和EphB4(4.58±0.82)较Sham组(CDs蛋白:0.62±0.06,MVD:1.50±1.04,ephrinB2:1.28±0.24,EphB4:1.21±0.22)均明显增多(P ＜0.05);EPO治疗组CD34蛋白(0.98±0.07)与MVD(4.71±1.38)表达较HI组(CD34蛋白:0.85±0.06,MVD:3.14±1.21)增多(P＜0.05),但ephrinB2和EphB4表达未见增多.结论 未成熟鼠脑HI损伤后出现了内源性的血管新生反应,且rh-EPO可进一步促进该反应.
目的 探討人重組促紅細胞生成素(rh-EPO)在早產鼠腦室週圍白質損傷模型鼠腦白質中的促血管新生作用.方法 選取齣生3日齡SD大鼠,隨機數字錶法分為假手術(Sham)組,缺氧缺血(HI)組和EPO治療組.HI組和EPO治療組幼鼠結扎右側頸總動脈併吸入60 mL/L的氮氧混閤氣體4h,Sham組幼鼠僅遊離右側頸總動脈.EPO治療組術後即經腹腔給予大劑量rh-EPO(5 U/g)1次,Sham組和HI組給予等量的9 g/L鹽水.採用免疫組織化學、Western blot和實時熒光定量PCR(qRT-PCR)方法檢測術後48 h及96 h大鼠腦白質中微血管密度(MVD)、CD34蛋白、動靜脈標誌物(ephrinB2和EphB4)錶達情況.結果 術後48 h,HI組CD34蛋白(0.54±0.05)較Sham組(0.42±0.05)增加(P＜0.05),但HI組MVD、ephrinB2和EphB4與Sham組比較錶達未見明顯改變(P ＞0.05);EPO治療組CD34蛋白(0.74±0.07)、ephrinB2(2.07±0.58)和EphB4 (2.13±0.44)錶達則較HI組(CD34蛋白:0.54±0.05,ephrinB2:0.70±0.23,EphB4:0.67±0.07)均明顯增加(P均＜0.05),但是MVD未見明顯增多(P＞0.05).術後96 h,HI組CD34蛋白(0.85±0.06)、MVD(3.14±1.21)、ephrinB2(7.51 ±1.89)和EphB4(4.58±0.82)較Sham組(CDs蛋白:0.62±0.06,MVD:1.50±1.04,ephrinB2:1.28±0.24,EphB4:1.21±0.22)均明顯增多(P ＜0.05);EPO治療組CD34蛋白(0.98±0.07)與MVD(4.71±1.38)錶達較HI組(CD34蛋白:0.85±0.06,MVD:3.14±1.21)增多(P＜0.05),但ephrinB2和EphB4錶達未見增多.結論 未成熟鼠腦HI損傷後齣現瞭內源性的血管新生反應,且rh-EPO可進一步促進該反應.
목적 탐토인중조촉홍세포생성소(rh-EPO)재조산서뇌실주위백질손상모형서뇌백질중적촉혈관신생작용.방법 선취출생3일령SD대서,수궤수자표법분위가수술(Sham)조,결양결혈(HI)조화EPO치료조.HI조화EPO치료조유서결찰우측경총동맥병흡입60 mL/L적담양혼합기체4h,Sham조유서부유리우측경총동맥.EPO치료조술후즉경복강급여대제량rh-EPO(5 U/g)1차,Sham조화HI조급여등량적9 g/L염수.채용면역조직화학、Western blot화실시형광정량PCR(qRT-PCR)방법검측술후48 h급96 h대서뇌백질중미혈관밀도(MVD)、CD34단백、동정맥표지물(ephrinB2화EphB4)표체정황.결과 술후48 h,HI조CD34단백(0.54±0.05)교Sham조(0.42±0.05)증가(P＜0.05),단HI조MVD、ephrinB2화EphB4여Sham조비교표체미견명현개변(P ＞0.05);EPO치료조CD34단백(0.74±0.07)、ephrinB2(2.07±0.58)화EphB4 (2.13±0.44)표체칙교HI조(CD34단백:0.54±0.05,ephrinB2:0.70±0.23,EphB4:0.67±0.07)균명현증가(P균＜0.05),단시MVD미견명현증다(P＞0.05).술후96 h,HI조CD34단백(0.85±0.06)、MVD(3.14±1.21)、ephrinB2(7.51 ±1.89)화EphB4(4.58±0.82)교Sham조(CDs단백:0.62±0.06,MVD:1.50±1.04,ephrinB2:1.28±0.24,EphB4:1.21±0.22)균명현증다(P ＜0.05);EPO치료조CD34단백(0.98±0.07)여MVD(4.71±1.38)표체교HI조(CD34단백:0.85±0.06,MVD:3.14±1.21)증다(P＜0.05),단ephrinB2화EphB4표체미견증다.결론 미성숙서뇌HI손상후출현료내원성적혈관신생반응,차rh-EPO가진일보촉진해반응.
Objective To evaluate whether recombinant human erythropoietin (rh-EPO) could increase the angiogenic responses in preterm rat models with periventricular white matter damage (PWMD).Methods Three-day postnatal rats were divided into 3 groups randomly:the sham group,the hypoxic-ischemic (HI) group and the recombinant human erythropoietin(rh-EPO)treatment group.Rat pups underwent permanent ligation of the right common carotid artery followed by 60 mL/L O2 for 4 h or sham operation and normoxic exposure.Immediately after the HI,rats received a single intraperitoneal injection of rh-EPO (5 U/g)or saline.Angiogenesis-related cells (CD34 + cells),microvessel density (MVD)and arteriovenous related genes (ephrinB2 and EphB4)were examined at 48 h and 96 h after operation.Results At 48 h after operation,the proteins of CD34 in HI rats increased compared with the sham rats (HI group vs Sham group:0.54 ± 0.05 vs 0.42 ± 0.05,P ＜ 0.05).However,the MVD,the mRNA of ephrinB2 and EphB4 did not change (P ＞ 0.05).The proteins of CD34,the mRNA of ephrinB2 and EphB4 increased after rh-EPO treatment compared with HI rats.However,the MVD did not increase.As the proteins of CD34 increased further at 96 h after operation (HI group vs Sham group:0.85 ± 0.06 vs 0.62 ± 0.06,P ＜ 0.05),the MVD (3.14 ± 1.21 vs 1.50 ± 1.04),ephrinB2 (7.51 ± 1.89 vs 1.28 ± 0.24) and EphB4 (4.58 ± 0.82 vs 1.21 ± 0.22) also increased (all P ＜ 0.05).And the proteins of CD34 increased after administration of rh-EPO(EPO group vs HI group:0.98 ± 0.07 vs 0.85 ± 0.06,P ＜0.05) and MVD(EPO group vs HI group:4.71 ± 1.38 vs 3.14 ± 1.21,P ＜0.05) were increased after administration of rh-EPO.However,the ephrinB2 and EphB4 did not increased after administration of rh-EPO at 96h time point.Conclusion Endogenous regenerative response is triggered in the damaged tissues and rh-EPO increases the angiogenesis in model rats with PWMD.
