中华实用儿科临床杂志
中華實用兒科臨床雜誌
중화실용인과림상잡지
Journal of Applied Clinical Pediatrics
2015年
10期
733-737
,共5页
董燕红%李士朋%乔艳红%宁雪%曾婷%俞桑洁%姚开虎%高薇%袁琳
董燕紅%李士朋%喬豔紅%寧雪%曾婷%俞桑潔%姚開虎%高薇%袁琳
동연홍%리사붕%교염홍%저설%증정%유상길%요개호%고미%원림
耐甲氧西林金黄色葡萄球菌%儿童%毒力%基因%表达
耐甲氧西林金黃色葡萄毬菌%兒童%毒力%基因%錶達
내갑양서림금황색포도구균%인동%독력%기인%표체
Methicillin-resistant Staphylococcus aureus%Child%Virulence%Gene%Expression
目的 探讨儿童耐甲氧西林金黄色葡萄球菌(MRSA)感染分离株的毒力特征.方法 收集2004年6月至2012年7月4家儿童医院分离的243株MRSA,采用PCR法对MRSA进行多位点序列分型(MLST);按随机分层抽样法对136株菌进行42种毒力基因及4种重要毒力基因hla、psmα、RNAⅢ和pvl的表达水平检测.结果 MRSA分离株大多数来源于肺炎和皮肤软组织感染(SSTIs),占88.1%(214/243株).243株菌检测到22种MLST,6种主要流行克隆是ST59、ST239、ST1、ST910、ST88和ST338.131株菌(96.3%)至少含有1种超抗原基因.所有菌株均携带黏附基因中的fnbA、clfA和spa.所有菌株均携带hla,psmα和RNAⅢ,pvl的携带率为39%.pvl在ST59、ST910、ST338分离株中的携带率较ST239、ST1、ST88高(P均<0.05).hla、smα、RNAⅢ在ST59中的表达水平较其他分型高(P均<0.05).社区获得性MRSA (CA-MRSA)分离株pvl的携带率高于医院获得性MRSA(HA-MRSA)分离株(48.8%比24.1%,x2 =8.356,P=0.004),但pvl表达水平比较差异无统计学意义(t=-0.777,P=0.441).CA-MRSA分离株hla、smα和RNAⅢ的表达水平较HA-MRSA分离株高(P均<0.05).肺炎分离株fnbB的携带率明显高于SSTIs分离株(27.5%比12.7%,x2 =4.050,P=0.044),而SSTIs分离株pvl的携带率明显高于肺炎分离株(52.7%比27.5%,x22=8.186,P=0.004).侵袭性感染分离株的hla表达水平较非侵袭性感染高(=2.433,P=0.0163).结论 儿童MRSA感染分离株的主要流行克隆间部分毒力基因携带存在差异.CA-MRSA较HA-MRSA高表达hla、psmα、RNAⅢ,尤其是ST59型,提示该型菌株可能具有较强的致病性.fnbB与肺炎、pvl与SSTIs可能有关.菌株高表达hla可能与侵袭性感染有关.
目的 探討兒童耐甲氧西林金黃色葡萄毬菌(MRSA)感染分離株的毒力特徵.方法 收集2004年6月至2012年7月4傢兒童醫院分離的243株MRSA,採用PCR法對MRSA進行多位點序列分型(MLST);按隨機分層抽樣法對136株菌進行42種毒力基因及4種重要毒力基因hla、psmα、RNAⅢ和pvl的錶達水平檢測.結果 MRSA分離株大多數來源于肺炎和皮膚軟組織感染(SSTIs),佔88.1%(214/243株).243株菌檢測到22種MLST,6種主要流行剋隆是ST59、ST239、ST1、ST910、ST88和ST338.131株菌(96.3%)至少含有1種超抗原基因.所有菌株均攜帶黏附基因中的fnbA、clfA和spa.所有菌株均攜帶hla,psmα和RNAⅢ,pvl的攜帶率為39%.pvl在ST59、ST910、ST338分離株中的攜帶率較ST239、ST1、ST88高(P均<0.05).hla、smα、RNAⅢ在ST59中的錶達水平較其他分型高(P均<0.05).社區穫得性MRSA (CA-MRSA)分離株pvl的攜帶率高于醫院穫得性MRSA(HA-MRSA)分離株(48.8%比24.1%,x2 =8.356,P=0.004),但pvl錶達水平比較差異無統計學意義(t=-0.777,P=0.441).CA-MRSA分離株hla、smα和RNAⅢ的錶達水平較HA-MRSA分離株高(P均<0.05).肺炎分離株fnbB的攜帶率明顯高于SSTIs分離株(27.5%比12.7%,x2 =4.050,P=0.044),而SSTIs分離株pvl的攜帶率明顯高于肺炎分離株(52.7%比27.5%,x22=8.186,P=0.004).侵襲性感染分離株的hla錶達水平較非侵襲性感染高(=2.433,P=0.0163).結論 兒童MRSA感染分離株的主要流行剋隆間部分毒力基因攜帶存在差異.CA-MRSA較HA-MRSA高錶達hla、psmα、RNAⅢ,尤其是ST59型,提示該型菌株可能具有較彊的緻病性.fnbB與肺炎、pvl與SSTIs可能有關.菌株高錶達hla可能與侵襲性感染有關.
목적 탐토인동내갑양서림금황색포도구균(MRSA)감염분리주적독력특정.방법 수집2004년6월지2012년7월4가인동의원분리적243주MRSA,채용PCR법대MRSA진행다위점서렬분형(MLST);안수궤분층추양법대136주균진행42충독력기인급4충중요독력기인hla、psmα、RNAⅢ화pvl적표체수평검측.결과 MRSA분리주대다수래원우폐염화피부연조직감염(SSTIs),점88.1%(214/243주).243주균검측도22충MLST,6충주요류행극륭시ST59、ST239、ST1、ST910、ST88화ST338.131주균(96.3%)지소함유1충초항원기인.소유균주균휴대점부기인중적fnbA、clfA화spa.소유균주균휴대hla,psmα화RNAⅢ,pvl적휴대솔위39%.pvl재ST59、ST910、ST338분리주중적휴대솔교ST239、ST1、ST88고(P균<0.05).hla、smα、RNAⅢ재ST59중적표체수평교기타분형고(P균<0.05).사구획득성MRSA (CA-MRSA)분리주pvl적휴대솔고우의원획득성MRSA(HA-MRSA)분리주(48.8%비24.1%,x2 =8.356,P=0.004),단pvl표체수평비교차이무통계학의의(t=-0.777,P=0.441).CA-MRSA분리주hla、smα화RNAⅢ적표체수평교HA-MRSA분리주고(P균<0.05).폐염분리주fnbB적휴대솔명현고우SSTIs분리주(27.5%비12.7%,x2 =4.050,P=0.044),이SSTIs분리주pvl적휴대솔명현고우폐염분리주(52.7%비27.5%,x22=8.186,P=0.004).침습성감염분리주적hla표체수평교비침습성감염고(=2.433,P=0.0163).결론 인동MRSA감염분리주적주요류행극륭간부분독력기인휴대존재차이.CA-MRSA교HA-MRSA고표체hla、psmα、RNAⅢ,우기시ST59형,제시해형균주가능구유교강적치병성.fnbB여폐염、pvl여SSTIs가능유관.균주고표체hla가능여침습성감염유관.
Obgective To investigate the virulence characteristics of methicillin-resistant Staphylococcus aureus(MRSA) isolates from Chinese children.Methods A total of 243 MRSA isolates were collected from 4 hospitals from June of 2004 to July of 2012.Multilocus sequence typing was analyzed by PCR.A total of 136 strains were selected from 6 major STs of 243 isolates based on stratified sampling.The carriages of 42 virulence genes were tested.Expression levels of hla,psmα and RNA Ⅲ genes were also detected,as well as pvl.Results Most of MRSA isolates were from pneumonia and skin and soft tissue infections (SSTIs),accounting for 88.1% (214/243 strains).Twenty-two sequence types were obtained,and 6 epidemic clones were ST59,ST239,ST1,ST910,ST88 and ST338.It was found that 96.3% strains(131/136 strains) contained at least one superantigen gene.All isolates were presented with fnbA,clfA and spa genes.All these 136 isolates all had hla,psmα and RNAⅢ.The percentage ofpvlgene was 39%.The pvl gene was more frequent in ST59,ST910,ST338 than in ST239,ST1,ST88(all P <0.05).The expression levels of hla,psmα and RNA Ⅲ were higher in ST59 than in other STs(all P <0.05).Thepvl gene was more associated with community acquired MRSA(CA-MRSA) than with hospital acquired MRSA(HA-MRSA) (48.8% vs 24.1%,x2 =8.356,P =0.004),but there was no statistically significant difference in their expression levels (t =-0.777,P =0.441).CA MRSA expressed hla,psma,and RNA Ⅲ in much higher levels (P < 0.05).The fnbB gene was more associated with pneumonia than with SSTIs (27.5% vs 12.7%,x2 =4.050,P =0.044),while the pvl gene was more associated with SSTIs than with pneumonia (52.7 % vs 27.5 %,x2 =8.186,P =0.004).Expression of hla in isolates from invasive infections was higher than that in isolates from non-invasive infections(t =2.433,P =0.0163).Conclusions The distribution of virulence genes among epidemic MRSA strains isolated from children was different.CA-MRSA expressed hla,psmα,and RNA Ⅲ in much higher levels,especially ST59 strains,which might lead to their enhanced pathogenicity.The fnbB gene might be associated with pneumonia,while the pvl gene is associated with SSTIs,and the hla gene might be associated with invasive infection.