CAJ | 학술논문

目的：探讨棉酚( gossypol)对胃癌细胞迁移能力的影响,并探讨相关机制。方法胃癌细胞经不同浓度棉酚处理后,MTT法检测细胞增殖能力,Transwell 小室实验检测细胞迁移能力, Western blot 法检测丝/苏氨酸激酶/β-链蛋白( Akt/β-catenin)信号通路活性及相关蛋白细胞周期蛋白D1(cyclin D1)、基质金属蛋白酶-2(MMP-2)、E-钙黏蛋白(E-cadherin)和波形蛋白( vimentin)的蛋白表达水平。结果MTT实验显示,棉酚对胃癌细胞具有增殖抑制作用,且随浓度增加而增大；Transwell小室实验显示棉酚对胃癌细胞迁移能力具有抑制作用；棉酚对胃癌细胞迁移能力的抑制率高于增殖抑制率；与对照组相比,棉酚可明显下调胃癌细胞的p-Akt、β-catenin、cyclin D1、MMP-2和vimentin蛋白表达水平,明显上调E-cadherin蛋白表达水平。结论棉酚通过下调Akt/β-catenin通路活性抑制胃癌细胞迁移。
목적：탐토면분( gossypol)대위암세포천이능력적영향,병탐토상관궤제。방법위암세포경불동농도면분처리후,MTT법검측세포증식능력,Transwell 소실실험검측세포천이능력, Western blot 법검측사/소안산격매/β-련단백( Akt/β-catenin)신호통로활성급상관단백세포주기단백D1(cyclin D1)、기질금속단백매-2(MMP-2)、E-개점단백(E-cadherin)화파형단백( vimentin)적단백표체수평。결과MTT실험현시,면분대위암세포구유증식억제작용,차수농도증가이증대；Transwell소실실험현시면분대위암세포천이능력구유억제작용；면분대위암세포천이능력적억제솔고우증식억제솔；여대조조상비,면분가명현하조위암세포적p-Akt、β-catenin、cyclin D1、MMP-2화vimentin단백표체수평,명현상조E-cadherin단백표체수평。결론면분통과하조Akt/β-catenin통로활성억제위암세포천이。
Aim To investigate the inhibitory effects of gossypol on migration in gastric carcinoma cell lines and its mechanisms. Methods Gastric carcinoma cells were treated with gossypol at different concentra-tions. The effects of gossypol on cells proliferation were measured using the MTT assay. The migration of gas-tric carcinoma cells was detected by transwell assay. The activation of Akt/β-catenin pathway and the ex-pressions of pathway related proteins ( p-Akt,β-cate-nin, cyclin D1, MMP-2, E-cadherin and vimentin ) were detected by Western blot. Results Gossypol treatment could significantly inhibit the proliferation of gastric carcinoma cells in a dose-dependent manner. Transwell assay showed that the migration ability of <br> gastric carcinoma cells was significantly decreased. The inhibitory effect of gossypol on cells migration was more significant than the effect of gossypol on cells prolifera-tion. Compared with the control group, treatment with gossypol significantly suppressed the expressions of p-Akt,β-catenin, cyclin D1, MMP-2 and vimentin pro-tein, whereas the expression of E-cadherin was signifi-cantly up-regulated in gastric carcinoma cells in a dose-dependent manner. Conclusion These results demonstrate that gossypol represses cell migration of gastric carcinoma cells through the down-regulation of the activity of Akt/β-catenin pathway.