CAJ | 학술논문

目的：探讨mmLDL对小鼠肠系膜动脉α1受体的作用。方法小鼠尾静脉注射mmLDL,微血管肌张力描记仪观察NA引起的小鼠肠系膜动脉收缩量效曲线变化, RT-PCR、Western blot 检测α1受体及α2受体表达。结果mmLDL引起NA收缩量效曲线明显增强,表现为Emax值由生理盐水( NS )组的(120．75±3．44)%上升为(161．00±6．87)%(P<0．01),pEC50值由NS组的(5．65±0．05)上升为(6．20±0．08)(P<0．01)。α1受体拮抗剂哌唑嗪引起量效曲线的明显右移,mmLDL引起α1受体mRNA水平、蛋白表达明显增加,对α2受体表达基本没有影响。结论尾静脉注射mmLDL上调小鼠肠系膜动脉α1受体。
목적：탐토mmLDL대소서장계막동맥α1수체적작용。방법소서미정맥주사mmLDL,미혈관기장력묘기의관찰NA인기적소서장계막동맥수축량효곡선변화, RT-PCR、Western blot 검측α1수체급α2수체표체。결과mmLDL인기NA수축량효곡선명현증강,표현위Emax치유생리염수( NS )조적(120．75±3．44)%상승위(161．00±6．87)%(P<0．01),pEC50치유NS조적(5．65±0．05)상승위(6．20±0．08)(P<0．01)。α1수체길항제고서진인기량효곡선적명현우이,mmLDL인기α1수체mRNA수평、단백표체명현증가,대α2수체표체기본몰유영향。결론미정맥주사mmLDL상조소서장계막동맥α1수체。
Aim To investigate the effects of mmLDL on the up-regulation ofα1 receptors in moues mesenter- <br> ic arteries. Methods Mice tail intravenous injection of mmLDL was used . Vitro sensitive myograph was empl- <br> oyed to examine Noradrenaline ( NA) induced vascular contraction on mice mesenteric artery, and the mRNA and protein expressions ofα1 andα2 receptors were an-alyzed by real-time PCR and Western blot, respective-ly. Results mmLDL significantly increased NA in-duced concentration-contractile curve, and the data of Emax and pEC50 were from ( 122. 61 ± 9. 40 )% and (5. 65 ± 0. 05 ) in normal saline ( NS ) group to (161. 01 ± 6. 90 )% and ( 6. 20 ± 0. 08 ) in mmLDL group (P <0. 01, P <0. 01), respectively. The α1 adrenoceptor antagonist prazosin shifted the concentra- <br> tion-contractile curve induced by NA towards right. Af-ter using mmLDL, the mRNA and protein levels of α1 adrenoceptor were significantly increased, but the mR-NA and protein levels of α2 adrenoceptor were not changed. Conclusion Tail intravenous injection of mmLDL enhances the vascular expressions of α1 adre-noceptors and the contractile effects mediated byα1 ad-renoceptors.