中国药理学通报
中國藥理學通報
중국약이학통보
CHINESE PHARMACOLOGICAL BULLETIN
2015年
6期
815-820,821
,共7页
陈力%薛瑞%于能江%王玉露%胡晓旭%仇志坤%赵楠%李云峰%徐江平%张有志
陳力%薛瑞%于能江%王玉露%鬍曉旭%仇誌坤%趙楠%李雲峰%徐江平%張有誌
진력%설서%우능강%왕옥로%호효욱%구지곤%조남%리운봉%서강평%장유지
小补心汤总黄酮%抗抑郁%获得性无助模型%行为%HPA轴%脑源性神经营养因子
小補心湯總黃酮%抗抑鬱%穫得性無助模型%行為%HPA軸%腦源性神經營養因子
소보심탕총황동%항억욱%획득성무조모형%행위%HPA축%뇌원성신경영양인자
XBXT-2%antidepressant effect%learned helplessness model%behavior%HPA axis%BDNF
目的：采用小鼠获得性无助模型评价小补心汤总黄酮(XBXT-2)的抗抑郁作用,并探究在应激状态下,XBXT-2对下丘脑-垂体-肾上腺轴( HPA)功能的调节作用。方法采用电脑程序控制的穿梭箱条件反射系统,给予不可逃避的足底电击诱导获得性无助模型,经过穿梭箱回避程序筛选,将成功诱导无助行为的小鼠分为模型组、度洛西汀( Dlx,20 mg·kg-1)组和XBXT-2(25,50 mg·kg-1)组,灌胃给药4 d,每日采用条件性回避反应测试程序,测定小鼠的逃避失败次数和逃避潜伏期。行为学检测结束后,采用酶联免疫吸附试验法检测血清皮质酮( CORT)和促肾上腺皮质激素( ACTH)水平、蛋白印迹法检测海马糖皮质激素受体( GRα/β)蛋白和脑源性神经营养因子( BDNF )的表达水平。结果亚慢性给予XBXT-2(25,50 mg·kg-1)可明显减少获得性无助小鼠逃避失败次数和(或)逃避失败潜伏期,在行为学有效基础上,亦可明显降低获得性无助小鼠血清CORT和ACTH水平,上调海马GRα/β蛋白和BDNF的表达。结论 XBTX-2
目的：採用小鼠穫得性無助模型評價小補心湯總黃酮(XBXT-2)的抗抑鬱作用,併探究在應激狀態下,XBXT-2對下丘腦-垂體-腎上腺軸( HPA)功能的調節作用。方法採用電腦程序控製的穿梭箱條件反射繫統,給予不可逃避的足底電擊誘導穫得性無助模型,經過穿梭箱迴避程序篩選,將成功誘導無助行為的小鼠分為模型組、度洛西汀( Dlx,20 mg·kg-1)組和XBXT-2(25,50 mg·kg-1)組,灌胃給藥4 d,每日採用條件性迴避反應測試程序,測定小鼠的逃避失敗次數和逃避潛伏期。行為學檢測結束後,採用酶聯免疫吸附試驗法檢測血清皮質酮( CORT)和促腎上腺皮質激素( ACTH)水平、蛋白印跡法檢測海馬糖皮質激素受體( GRα/β)蛋白和腦源性神經營養因子( BDNF )的錶達水平。結果亞慢性給予XBXT-2(25,50 mg·kg-1)可明顯減少穫得性無助小鼠逃避失敗次數和(或)逃避失敗潛伏期,在行為學有效基礎上,亦可明顯降低穫得性無助小鼠血清CORT和ACTH水平,上調海馬GRα/β蛋白和BDNF的錶達。結論 XBTX-2
목적：채용소서획득성무조모형평개소보심탕총황동(XBXT-2)적항억욱작용,병탐구재응격상태하,XBXT-2대하구뇌-수체-신상선축( HPA)공능적조절작용。방법채용전뇌정서공제적천사상조건반사계통,급여불가도피적족저전격유도획득성무조모형,경과천사상회피정서사선,장성공유도무조행위적소서분위모형조、도락서정( Dlx,20 mg·kg-1)조화XBXT-2(25,50 mg·kg-1)조,관위급약4 d,매일채용조건성회피반응측시정서,측정소서적도피실패차수화도피잠복기。행위학검측결속후,채용매련면역흡부시험법검측혈청피질동( CORT)화촉신상선피질격소( ACTH)수평、단백인적법검측해마당피질격소수체( GRα/β)단백화뇌원성신경영양인자( BDNF )적표체수평。결과아만성급여XBXT-2(25,50 mg·kg-1)가명현감소획득성무조소서도피실패차수화(혹)도피실패잠복기,재행위학유효기출상,역가명현강저획득성무조소서혈청CORT화ACTH수평,상조해마GRα/β단백화BDNF적표체。결론 XBTX-2
Aim To investigate the effect of the total flaconoids extracted from Xiaobuxin-Tan g ( XBXT-2 ) <br> on the hyperactivity of hypothalamic-pituitary-adrenal axis in mouse learned helplessness model. Methods Learned helplessness was induced by inescapable foot shock stress over 1h session for 5 days. After screen-ing, we divided learned helplessness mice into five groups:IS, inescapable shock;Dlx, dulxetine(20 mg ·kg-1);XBXT-2(25,50 mg·kg-1). Latency to es-cape shocks and escape failure had been recorded. During the test, Dlx(20 mg·kg-1 ) and XBXT-2(25, 50 mg·kg-1 ) were administered intragastrically once daily for four days. Serum corticosterone level and ad-renocorticotropic hormone ( ACTH ) level were meas-ured by ELISA, and expression of glucocorticoids re-ceptor ( GR) α/β and brain-derived neurotrophic fac-tor ( BDNF ) in hippocampus was determined using Western blot method. Results XBXT-2 (25,50 mg· <br> kg-1 ) or duloxetine treatment showed antidepressant effect in mouse learned helplessness model, as demon-strated by the decreased escape failure and escape la-tency. Our ELISA results showed that XBXT-2 or du-loxetine significantly decreased serum corticosterone level and its upstream stress hormone ACTH level in learned helplessness mice. Furthermore, Western blot result demonstrated XBXT-2 treatment increased GRs and BDNF expression in hippocampus. Conclusions XBXT-2 produces significant antidepressant effect on learned helplessness mice. In addition, the modulation of HPA axis produced by XBXT-2 may be important mechanism underlying its antidepressant-like effect in mouse learned helplessness model.
