CAJ | 학술논문

目的：探讨中药单体黄芩苷对全脑缺血/再灌注损伤大鼠认知功能的影响及其作用机制。方法将60只♂ SD大鼠,随机分为假手术组(S组)、全脑缺血/再灌注组(I/R组)、全脑缺血/再灌注+黄芩苷组( I/RB组),每组20只。全脑缺血/再灌注模型采用双侧颈总动脉夹闭合并低血压。I/RB组大鼠造模后12 h行黄芩苷(100 mg · kg-1)灌胃,每天两次,连续7d。 S组及I/R组大鼠在相同时间点给予相应体积的生理盐水灌胃。造模7 d后,采用Morris水迷宫检测空间学习记忆能力,BrdU免疫组化标记脑内神经前体细胞的增殖,Western blot检测脑组织COX-2蛋白的表达。结果与I/R组比较,I/RB组大鼠的学习记忆功能明显改善(P<0．05),海马齿状回(DG)和侧脑室室管膜下区(SVZ) BrdU阳性细胞数目明显增加( P<0．01),脑组织COX-2蛋白的表达明显下降( P<0．01)。结论黄芩苷能明显改善全脑缺血/再灌注大鼠的认知功能,机制可能与抑制脑组织COX-2蛋白的表达,促进脑内神经前体细胞增殖有关。
목적：탐토중약단체황금감대전뇌결혈/재관주손상대서인지공능적영향급기작용궤제。방법장60지♂ SD대서,수궤분위가수술조(S조)、전뇌결혈/재관주조(I/R조)、전뇌결혈/재관주+황금감조( I/RB조),매조20지。전뇌결혈/재관주모형채용쌍측경총동맥협폐합병저혈압。I/RB조대서조모후12 h행황금감(100 mg · kg-1)관위,매천량차,련속7d。 S조급I/R조대서재상동시간점급여상응체적적생리염수관위。조모7 d후,채용Morris수미궁검측공간학습기억능력,BrdU면역조화표기뇌내신경전체세포적증식,Western blot검측뇌조직COX-2단백적표체。결과여I/R조비교,I/RB조대서적학습기억공능명현개선(P<0．05),해마치상회(DG)화측뇌실실관막하구(SVZ) BrdU양성세포수목명현증가( P<0．01),뇌조직COX-2단백적표체명현하강( P<0．01)。결론황금감능명현개선전뇌결혈/재관주대서적인지공능,궤제가능여억제뇌조직COX-2단백적표체,촉진뇌내신경전체세포증식유관。
Aim To investigate the effects of baicalin on cognitive function in global cerebral ischemia reper-fusion rats, and the probable mechanism involved. Methods Sixty male Sprague-Dawley(SD) rats were randomly divided into Sham operation group ( S group) , global cerebral ischemia reperfusion group ( I/R group) , global cerebral ischemia reperfusion + ba-icalin treatment group ( I/RB group) , twenty in each. Model was induced via the bilateral occlusion of the <br> common carotid arteries plus hemorrhagic hypotension. 12 h after reperfusion, rats in I/RB group were given baicalin (100 mg·kg-1 ) saline solution by intragas-tric administration twice per day for 7 days. Rats in S group and I/R group were given the corresponding dose of saline infusion at the same time. Morris water maze test was employed to detect spatial learning and memo-ry. BrdU immunohistochemistry was used to detect the proliferation of neural precursor cells ( NPCs ) in the <br> brain. Expression of COX-2 in the brain tissue was measured by Western blot. Results Compared to I/R group, baicalin improved spatial learning and memory damage ( P <0. 05 ) , increased the number of BrdU-positive cells in the DG and SVZ ( P<0. 01 ) , and re-duced the expression of COX-2 in the brain ( P <0. 01 ) . Conclusions Baicalin could ameliorate cog- <br> nitive function in the rats with global cerebral ischemia reperfusion, which might be associated with its inhibi-tory effects on the expression of COX-2 , thereby in-creasing the proliferation of NPCs in the brain.