中国药理学通报
中國藥理學通報
중국약이학통보
CHINESE PHARMACOLOGICAL BULLETIN
2015年
6期
790-794,795
,共6页
郑智茵%尹利明%庄海峰%成志%赵燕娜%余潇苓%高瑞兰
鄭智茵%尹利明%莊海峰%成誌%趙燕娜%餘瀟苓%高瑞蘭
정지인%윤리명%장해봉%성지%조연나%여소령%고서란
人参二醇组皂苷%再生障碍性贫血%免疫调节%T细胞亚群%小鼠%实验研究
人參二醇組皂苷%再生障礙性貧血%免疫調節%T細胞亞群%小鼠%實驗研究
인삼이순조조감%재생장애성빈혈%면역조절%T세포아군%소서%실험연구
panaxdiol saponins component%aplastic a-nemia%immune regulation%T cell subsets%mice%ex-perimental studies
目的:观察自行提取分离的中药新药人参二醇组皂苷提取物( PDS-C)对再生障碍性贫血(再障)小鼠促进造血和调节免疫的作用。方法 BALB/c小鼠经亚致死量照射后输入DBA/2小鼠的淋巴细胞,制成免疫介导型再障模型。实验分6组,正常小鼠、模型组、PDS-C低、中和高剂量治疗组及环孢素阳性药物对照组,均灌胃给药15 d。检测外周血象、骨髓病理检查造血组织增生状况、脾脏T细胞亚群比例,及T细胞分化相关T-bet、GATA-3和FOXP3转录调控蛋白的表达水平。结果 PDS-C治疗再障小鼠的疗效明显,中、高剂量组的外周血红蛋白、白细胞和血小板计数均明显高于模型组,骨髓造血组织增生状况也明显优于模型组。 PDS-C升高Th2细胞和调节性T细胞( Treg)的比例,降低Th1细胞比例,并上调GATA-3和FOXP3蛋白及下调T-bet蛋白的表达。结论 PDS-C有效地促进造血,改善再障的骨髓抑制,加快造血功能恢复而升高外周血象;同时通过恢复再障小鼠失衡的 Th1/Th2/Treg 细胞比例而参与调节免疫,该文为PDS-C的临床应用提供实验依据。
目的:觀察自行提取分離的中藥新藥人參二醇組皂苷提取物( PDS-C)對再生障礙性貧血(再障)小鼠促進造血和調節免疫的作用。方法 BALB/c小鼠經亞緻死量照射後輸入DBA/2小鼠的淋巴細胞,製成免疫介導型再障模型。實驗分6組,正常小鼠、模型組、PDS-C低、中和高劑量治療組及環孢素暘性藥物對照組,均灌胃給藥15 d。檢測外週血象、骨髓病理檢查造血組織增生狀況、脾髒T細胞亞群比例,及T細胞分化相關T-bet、GATA-3和FOXP3轉錄調控蛋白的錶達水平。結果 PDS-C治療再障小鼠的療效明顯,中、高劑量組的外週血紅蛋白、白細胞和血小闆計數均明顯高于模型組,骨髓造血組織增生狀況也明顯優于模型組。 PDS-C升高Th2細胞和調節性T細胞( Treg)的比例,降低Th1細胞比例,併上調GATA-3和FOXP3蛋白及下調T-bet蛋白的錶達。結論 PDS-C有效地促進造血,改善再障的骨髓抑製,加快造血功能恢複而升高外週血象;同時通過恢複再障小鼠失衡的 Th1/Th2/Treg 細胞比例而參與調節免疫,該文為PDS-C的臨床應用提供實驗依據。
목적:관찰자행제취분리적중약신약인삼이순조조감제취물( PDS-C)대재생장애성빈혈(재장)소서촉진조혈화조절면역적작용。방법 BALB/c소서경아치사량조사후수입DBA/2소서적림파세포,제성면역개도형재장모형。실험분6조,정상소서、모형조、PDS-C저、중화고제량치료조급배포소양성약물대조조,균관위급약15 d。검측외주혈상、골수병리검사조혈조직증생상황、비장T세포아군비례,급T세포분화상관T-bet、GATA-3화FOXP3전록조공단백적표체수평。결과 PDS-C치료재장소서적료효명현,중、고제량조적외주혈홍단백、백세포화혈소판계수균명현고우모형조,골수조혈조직증생상황야명현우우모형조。 PDS-C승고Th2세포화조절성T세포( Treg)적비례,강저Th1세포비례,병상조GATA-3화FOXP3단백급하조T-bet단백적표체。결론 PDS-C유효지촉진조혈,개선재장적골수억제,가쾌조혈공능회복이승고외주혈상;동시통과회복재장소서실형적 Th1/Th2/Treg 세포비례이삼여조절면역,해문위PDS-C적림상응용제공실험의거。
Aim To observe the effects of panaxdiol saponins component ( PDS-C) extracted and isolated <br> from Chinese ginseng herb as new Chinese patent med-icine on the promotion of hematopoiesis and the regula-tion of the immune system in treating mice models with aplastic anemia ( AA ) . Methods For preparation of immune mediated AA models, BALB/c mice were ex-posed to sublethal doses of 5. 0 Gy γ radiation, fol-lowed by transplanted lymphocytes from DBA/2 donor mice. The mice models were divided into six groups in-cluding normal control, AA model, PDS-C treated groups with lower, medium and higher dosages, cy-closporine ( CsA) as positive drug control. Both PDS-C and CsA were administered by gastrogavage for 15 days. The peripheral blood cells counts and bone mar-row pathological examination were tested, the percenta-ges of Th1/Th2/Treg cells from spleen were measured, the protein expression levels of T-bet, GATA-3 and FOXP3 transcription factors in spleen cells were detec-ted. Results Curative effect of PDS-C on treating AA <br> mice was satisfactory. The peripheral hemoglobin, white blood cells and platelet counts in PDS-C groups with medium and higher doses were significantly higher than those in model control. Meanwhile, PDS-C ele-vated the percentages of Th2 cells and Treg cells, but decreased the percentage of Th1 cells, as well as up-regulated the GATA-3 , FOXP3 and down-regulated the T-bet protein levels. Conclusion PDS-C possesses the activities of promoting hematopoiesis obviously. It can improve marrow myelosuppression, enhance the re-covery of hematopoiestic function, and elevate the pe-ripheral blood cells counts. PDS-C also pays its immu-noregulatory efficacy though recovering from unbal-anced Th1/Th2/Treg cells in treating immune media-ted AA mice.