CAJ | 학술논문

目的：观察自行提取分离的中药新药人参二醇组皂苷提取物( PDS-C)对再生障碍性贫血(再障)小鼠促进造血和调节免疫的作用。方法 BALB/c小鼠经亚致死量照射后输入DBA/2小鼠的淋巴细胞，制成免疫介导型再障模型。实验分6组，正常小鼠、模型组、PDS-C低、中和高剂量治疗组及环孢素阳性药物对照组，均灌胃给药15 d。检测外周血象、骨髓病理检查造血组织增生状况、脾脏T细胞亚群比例，及T细胞分化相关T-bet、GATA-3和FOXP3转录调控蛋白的表达水平。结果 PDS-C治疗再障小鼠的疗效明显，中、高剂量组的外周血红蛋白、白细胞和血小板计数均明显高于模型组，骨髓造血组织增生状况也明显优于模型组。 PDS-C升高Th2细胞和调节性T细胞( Treg)的比例，降低Th1细胞比例，并上调GATA-3和FOXP3蛋白及下调T-bet蛋白的表达。结论 PDS-C有效地促进造血，改善再障的骨髓抑制，加快造血功能恢复而升高外周血象；同时通过恢复再障小鼠失衡的 Th1/Th2/Treg 细胞比例而参与调节免疫，该文为PDS-C的临床应用提供实验依据。
목적：관찰자행제취분리적중약신약인삼이순조조감제취물( PDS-C)대재생장애성빈혈(재장)소서촉진조혈화조절면역적작용。방법 BALB/c소서경아치사량조사후수입DBA/2소서적림파세포，제성면역개도형재장모형。실험분6조，정상소서、모형조、PDS-C저、중화고제량치료조급배포소양성약물대조조，균관위급약15 d。검측외주혈상、골수병리검사조혈조직증생상황、비장T세포아군비례，급T세포분화상관T-bet、GATA-3화FOXP3전록조공단백적표체수평。결과 PDS-C치료재장소서적료효명현，중、고제량조적외주혈홍단백、백세포화혈소판계수균명현고우모형조，골수조혈조직증생상황야명현우우모형조。 PDS-C승고Th2세포화조절성T세포( Treg)적비례，강저Th1세포비례，병상조GATA-3화FOXP3단백급하조T-bet단백적표체。결론 PDS-C유효지촉진조혈，개선재장적골수억제，가쾌조혈공능회복이승고외주혈상；동시통과회복재장소서실형적 Th1/Th2/Treg 세포비례이삼여조절면역，해문위PDS-C적림상응용제공실험의거。
Aim To observe the effects of panaxdiol saponins component ( PDS-C) extracted and isolated <br> from Chinese ginseng herb as new Chinese patent med-icine on the promotion of hematopoiesis and the regula-tion of the immune system in treating mice models with aplastic anemia ( AA ) . Methods For preparation of immune mediated AA models, BALB/c mice were ex-posed to sublethal doses of 5. 0 Gy γ radiation, fol-lowed by transplanted lymphocytes from DBA/2 donor mice. The mice models were divided into six groups in-cluding normal control, AA model, PDS-C treated groups with lower, medium and higher dosages, cy-closporine ( CsA) as positive drug control. Both PDS-C and CsA were administered by gastrogavage for 15 days. The peripheral blood cells counts and bone mar-row pathological examination were tested, the percenta-ges of Th1/Th2/Treg cells from spleen were measured, the protein expression levels of T-bet, GATA-3 and FOXP3 transcription factors in spleen cells were detec-ted. Results Curative effect of PDS-C on treating AA <br> mice was satisfactory. The peripheral hemoglobin, white blood cells and platelet counts in PDS-C groups with medium and higher doses were significantly higher than those in model control. Meanwhile, PDS-C ele-vated the percentages of Th2 cells and Treg cells, but decreased the percentage of Th1 cells, as well as up-regulated the GATA-3 , FOXP3 and down-regulated the T-bet protein levels. Conclusion PDS-C possesses the activities of promoting hematopoiesis obviously. It can improve marrow myelosuppression, enhance the re-covery of hematopoiestic function, and elevate the pe-ripheral blood cells counts. PDS-C also pays its immu-noregulatory efficacy though recovering from unbal-anced Th1/Th2/Treg cells in treating immune media-ted AA mice.