CAJ | 학술논문

目的：分析比较 BALB／c 和 CBA 小鼠局部淋巴结试验结果。方法将已知致敏性的4种化学物（2，4－二硝基氯苯、丁子香酚、己基肉桂醛和异丙醇）和未知致敏性的3种化妆品对 BALB／c 和 CBA 小鼠连续染毒3 d，第5天腹腔注射示踪剂 BrdU，第6天分离颌下淋巴结制取单细胞悬液，用 ELISA 试盒检测细胞增殖。结果2，4－二硝基氯苯、丁子香酚、己基肉桂醛和3号烫发剂两品系结果均呈致敏阳性，3种化学物 EC1．6值分别为0．08％（极强）、4．02％（中）、6．68％（中）（BALB／c）和0．07％（极强）、6．08％（中）、8．89％（中）（CBA）；异丙醇、1和2号化妆品两品系结果均为致敏阴性，SI 均小于1．6。结论两品系小鼠局部淋巴结试验结果较一致，BALB／c可替代 CBA 小鼠应用于化学物和化妆品的致敏性评价。
목적：분석비교 BALB／c 화 CBA 소서국부림파결시험결과。방법장이지치민성적4충화학물（2，4－이초기록분、정자향분、기기육계철화이병순）화미지치민성적3충화장품대 BALB／c 화 CBA 소서련속염독3 d，제5천복강주사시종제 BrdU，제6천분리합하림파결제취단세포현액，용 ELISA 시합검측세포증식。결과2，4－이초기록분、정자향분、기기육계철화3호탕발제량품계결과균정치민양성，3충화학물 EC1．6치분별위0．08％（겁강）、4．02％（중）、6．68％（중）（BALB／c）화0．07％（겁강）、6．08％（중）、8．89％（중）（CBA）；이병순、1화2호화장품량품계결과균위치민음성，SI 균소우1．6。결론량품계소서국부림파결시험결과교일치，BALB／c가체대 CBA 소서응용우화학물화화장품적치민성평개。
Objective To analyze and compare results of local lymph node assays (LLNA) between BALB/c and CBA mice.Methods 4 chemicals (2,4-dinitrochlorobenzene, eugenol, hexyl cinnamic aldehyde and isopropanol) and 3 cosmetics were applied to the dorsum of both ears of Balb/c and CBA mice for three consecutive days.BrdU solution was injected inter-peritoneally on day 5.On day 6, the bilateral draining auricular lymph nodes were excised and made into single cell suspension, the lymph cell proliferation was measured by BrdU ELISA kit.Results 2,4-dinitrochlorobenzene, eugenol, hexyl cinnamic aldehyde and NO.3 perm agent pretended positive for both strains, EC1.6 values of three chemicals were found to be 0.08% (very strong), 4.02% (moderate), 6.68% (moderate) and 0.07% (very strong), 6.08% ( moderate), 8.89% ( moderate) for BALB/c and CBA mice respectively.Isopropanol, NO.1 and NO.2 cosmetics pretended to be non-sensitizers with SI <1.6 for both strains.Conclusion This study showed that BALB/c mouse was essentially equal to CBA for LLNA: BrdU-ELISA, which suggested that BALB/c mouse was a good alternative for CBA used in chemicals and cosmetics allergenic evaluation.