中国比较医学杂志
中國比較醫學雜誌
중국비교의학잡지
CHINESE JOURNAL OF COMPARATIVE MEDICINE
2015年
5期
20-23
,共4页
袁琳%周秉舵%王晓素%吴中华%孔婧%徐亭亭%王宏伟%朱生樑%方盛泉%周莉%刘春芳%张秀莲%王轶
袁琳%週秉舵%王曉素%吳中華%孔婧%徐亭亭%王宏偉%硃生樑%方盛泉%週莉%劉春芳%張秀蓮%王軼
원림%주병타%왕효소%오중화%공청%서정정%왕굉위%주생량%방성천%주리%류춘방%장수련%왕질
重症胰腺炎%逆行胆胰管注射法%牛磺胆酸钠%模型,动物
重癥胰腺炎%逆行膽胰管註射法%牛磺膽痠鈉%模型,動物
중증이선염%역행담이관주사법%우광담산납%모형,동물
Severe acute pancreatitis%The method of retrograde injection into the biliopancreatic duct%Sodium taurocholate%Model,animal
目的：为研究者选择合适的牛磺胆酸钠浓度来制备大鼠重症胰腺炎（severe acute pancreatitis，SAP） SAP 模型提供依据。方法将60只 SD 大鼠随机分为假手术组、1．5％浓度组、3．5％浓度组和5％浓度组，造模各组分别用1．5％、3．5％和5％牛磺胆酸钠按逆行胆胰管注射法制备 SAP 模型。术后统计各组大鼠的死亡率；检测血清淀粉酶、肿瘤坏死因子－α（ TNF －α）、白介素－6（ IL －6）水平；观测各组大鼠胰腺组织 HE 染色病理评分。结果5％浓度组死亡率较1．5％浓度组显著升高，血淀粉酶、肿瘤坏死因子－α（ TNF －α）、白介素－6（ IL －6）水平、出血和腺泡组织坏死的病理评分较1．5％浓度组和3．5％浓度组均有显著升高。结论5％的牛磺胆酸钠逆行胆胰管注射法能更好的制备 SAP 模型，且更符合 SAP 的生理、病理表现。
目的：為研究者選擇閤適的牛磺膽痠鈉濃度來製備大鼠重癥胰腺炎（severe acute pancreatitis，SAP） SAP 模型提供依據。方法將60隻 SD 大鼠隨機分為假手術組、1．5％濃度組、3．5％濃度組和5％濃度組，造模各組分彆用1．5％、3．5％和5％牛磺膽痠鈉按逆行膽胰管註射法製備 SAP 模型。術後統計各組大鼠的死亡率；檢測血清澱粉酶、腫瘤壞死因子－α（ TNF －α）、白介素－6（ IL －6）水平；觀測各組大鼠胰腺組織 HE 染色病理評分。結果5％濃度組死亡率較1．5％濃度組顯著升高，血澱粉酶、腫瘤壞死因子－α（ TNF －α）、白介素－6（ IL －6）水平、齣血和腺泡組織壞死的病理評分較1．5％濃度組和3．5％濃度組均有顯著升高。結論5％的牛磺膽痠鈉逆行膽胰管註射法能更好的製備 SAP 模型，且更符閤 SAP 的生理、病理錶現。
목적：위연구자선택합괄적우광담산납농도래제비대서중증이선염（severe acute pancreatitis，SAP） SAP 모형제공의거。방법장60지 SD 대서수궤분위가수술조、1．5％농도조、3．5％농도조화5％농도조，조모각조분별용1．5％、3．5％화5％우광담산납안역행담이관주사법제비 SAP 모형。술후통계각조대서적사망솔；검측혈청정분매、종류배사인자－α（ TNF －α）、백개소－6（ IL －6）수평；관측각조대서이선조직 HE 염색병리평분。결과5％농도조사망솔교1．5％농도조현저승고，혈정분매、종류배사인자－α（ TNF －α）、백개소－6（ IL －6）수평、출혈화선포조직배사적병리평분교1．5％농도조화3．5％농도조균유현저승고。결론5％적우광담산납역행담이관주사법능경호적제비 SAP 모형，차경부합 SAP 적생리、병리표현。
Objective to provide the evidence for inducing the SAP model in rats with proper concentration of sodium taurocholate.Methods 60 SD rats were divided into sham operated group, group of 1.5% in concentration, group of 3.5% in concentration and group of 5% in concentration randomly, while the SAP model was induced by the sodium taurocholate concentration of 1.5%,3.5% and 5% with the method of retrograde injection into the biliopancreatic duct.To calculate the mortality of different groups, measure the serum amylase, tumor necrosis factor -α(TNF -α) and interleukin -6 (IL -6),and to observe the pancreatic pathological scores of HE staining in rats.Results The mortality in group of 5% in concentration has a significant ascending compared with group of 1.5% in concentration, while the serum amylase, tumor necrosis factor -α(TNF -α) , interleukin -6( IL -6), pathological score of hemorrhage and acinar necrosis in group of 5% in concentration have a significant ascending compared with group of 1.5% in concentration and group of 3.5% in concentration.Conclusions A better SAP model may be induced by sodium taurocholate with the concentration of 5% by the method of retrograde injection into the biliopancreatic duct, which may accord with the physiological and pathological manifestation of SAP.
