中国比较医学杂志
中國比較醫學雜誌
중국비교의학잡지
CHINESE JOURNAL OF COMPARATIVE MEDICINE
2015年
5期
67-70
,共4页
李超%董浩迪%徐琳凯%王春雨%宋志琦%刘春法%李超斯%岳瑞超%程广宇%赵化佳%赵德明%尹晓敏%周向梅%杨利峰
李超%董浩迪%徐琳凱%王春雨%宋誌琦%劉春法%李超斯%嶽瑞超%程廣宇%趙化佳%趙德明%尹曉敏%週嚮梅%楊利峰
리초%동호적%서림개%왕춘우%송지기%류춘법%리초사%악서초%정엄우%조화가%조덕명%윤효민%주향매%양리봉
啮齿类实验动物%病理学诊断%器官病变%油红 -O 染色%PAS 染色
齧齒類實驗動物%病理學診斷%器官病變%油紅 -O 染色%PAS 染色
교치류실험동물%병이학진단%기관병변%유홍 -O 염색%PAS 염색
Laboratory rodents%Pathological diagnosis%Organ diseases%Oil red-ostain%PAS stain
目的：从病理学角度评价啮齿类实验动物的健康情况，为实验动物标准化饲养提供建议，确保相关科研结果的准确性。方法本文主要对2014年10月北京市15家实验动物单位的啮齿类实验动物病理学抽检情况进行统计分析。采集啮齿类实验动物的心、肝、脾、肺、肾、大肠和小肠后经甲醛钙液固定，通过石蜡切片 HE 染色、PAS 染色、冰冻切片油红 O 染色后于显微镜下观察。结果实验动物总体合格，但个别单位仍然存在问题。检出病变器官主要是肝脏和肺脏，肝脏病变检出率小鼠为6％，大鼠为2．5％，豚鼠为8．2％，地鼠未检出，肝脏病变表现为细胞肿胀，且经特殊染色确证部分为脂肪变性；肺脏病变检出率豚鼠为15．5％，其余未检出，肺脏病变表现为淤血、渗出及间质性肺炎。结论实验动物病理学检测基本健康，肝脏出现损伤可能与饲料有关；而肺脏病变可能与垫料及空气有关，而秋冬季雾霾及低温可能对实验动物健康有所影响。
目的：從病理學角度評價齧齒類實驗動物的健康情況，為實驗動物標準化飼養提供建議，確保相關科研結果的準確性。方法本文主要對2014年10月北京市15傢實驗動物單位的齧齒類實驗動物病理學抽檢情況進行統計分析。採集齧齒類實驗動物的心、肝、脾、肺、腎、大腸和小腸後經甲醛鈣液固定，通過石蠟切片 HE 染色、PAS 染色、冰凍切片油紅 O 染色後于顯微鏡下觀察。結果實驗動物總體閤格，但箇彆單位仍然存在問題。檢齣病變器官主要是肝髒和肺髒，肝髒病變檢齣率小鼠為6％，大鼠為2．5％，豚鼠為8．2％，地鼠未檢齣，肝髒病變錶現為細胞腫脹，且經特殊染色確證部分為脂肪變性；肺髒病變檢齣率豚鼠為15．5％，其餘未檢齣，肺髒病變錶現為淤血、滲齣及間質性肺炎。結論實驗動物病理學檢測基本健康，肝髒齣現損傷可能與飼料有關；而肺髒病變可能與墊料及空氣有關，而鞦鼕季霧霾及低溫可能對實驗動物健康有所影響。
목적：종병이학각도평개교치류실험동물적건강정황，위실험동물표준화사양제공건의，학보상관과연결과적준학성。방법본문주요대2014년10월북경시15가실험동물단위적교치류실험동물병이학추검정황진행통계분석。채집교치류실험동물적심、간、비、폐、신、대장화소장후경갑철개액고정，통과석사절편 HE 염색、PAS 염색、빙동절편유홍 O 염색후우현미경하관찰。결과실험동물총체합격，단개별단위잉연존재문제。검출병변기관주요시간장화폐장，간장병변검출솔소서위6％，대서위2．5％，돈서위8．2％，지서미검출，간장병변표현위세포종창，차경특수염색학증부분위지방변성；폐장병변검출솔돈서위15．5％，기여미검출，폐장병변표현위어혈、삼출급간질성폐염。결론실험동물병이학검측기본건강，간장출현손상가능여사료유관；이폐장병변가능여점료급공기유관，이추동계무매급저온가능대실험동물건강유소영향。
Objective To assess the health status of rodent laboratory animals by pathological diagnosis, our lab has being take apart in investigating the quality of laboratory animals in Beijing area for years and offer some advices for standardized breeding to ensure accurate results of scientific research.This paper focuses on the analysis of laboratory rodent samples that collected in October 2014.Methods We collected the heart, liver, spleen, lung, kidney, large intestine and small intestine, and put these organs into 10%Calcium formaldehyde solution for fixation, and then prepared into two different sections for optical microscopy observation including all paraffin specimens stained with H&E and the frozen sections stained with Oil Red-O and PAS.Results The vast majority of laboratory rodents were up to standard, but there still a problem in individual units.The main problem is liver and lung disease.The rate of Hepatocyte swellingis 6%(mouse), 2.5% (rat), 8.2% (guinea pig), moreover part of them were lipidosis, according to Oil Red-O stain.the mainly problem of lung is congestion ,edema and Interstitial pneumonia ,the detectable rate of pulmonarydiseases is 15.5%(guinea pig).Conclusions The vast majority of laboratory rodents were pathologically diagnosed as healthy animals.The liver disease may be caused by improper feeding.And disease of lung may led by haze, unqualified bedding and low temperature.
