中国比较医学杂志
中國比較醫學雜誌
중국비교의학잡지
CHINESE JOURNAL OF COMPARATIVE MEDICINE
2015年
5期
51-53,50
,共4页
徐玉环%徐艳峰%刘颖%黄澜%李彦红%韩云林%邓巍%许庆刚%秦川%朱华
徐玉環%徐豔峰%劉穎%黃瀾%李彥紅%韓雲林%鄧巍%許慶剛%秦川%硃華
서옥배%서염봉%류영%황란%리언홍%한운림%산외%허경강%진천%주화
视网膜血管铺片%联合消化法%抗原修复
視網膜血管鋪片%聯閤消化法%抗原脩複
시망막혈관포편%연합소화법%항원수복
Retinal vascular preparation%Combined digestion%Antigen retrieval
目的：探讨视网膜血管铺片技术的改进及在实验动物小鼠，大鼠，狗和猴中的应用。方法应用蛋白酶 K －胰蛋白酶联合消化法制备视网膜血管铺片，行 HE 及 PAS 染色观察血管形态，免疫组化方法观察 CD31及VEGF 的表达。结果用改进方法制备的视网膜血管铺片，血管网完整，无神经组织残留，可清晰显示血管走向。各级血管形态清晰，血管分支清楚完整，无断裂现象。血管内皮细胞形态清晰。 CD31在血管周细胞及内皮细胞均有表达；VEGF 主要在内皮细胞表达。结论改进的视网膜血管铺片技术可成功应用于不同实验动物，为视网膜血管性疾病的研究提供了重要途径。
目的：探討視網膜血管鋪片技術的改進及在實驗動物小鼠，大鼠，狗和猴中的應用。方法應用蛋白酶 K －胰蛋白酶聯閤消化法製備視網膜血管鋪片，行 HE 及 PAS 染色觀察血管形態，免疫組化方法觀察 CD31及VEGF 的錶達。結果用改進方法製備的視網膜血管鋪片，血管網完整，無神經組織殘留，可清晰顯示血管走嚮。各級血管形態清晰，血管分支清楚完整，無斷裂現象。血管內皮細胞形態清晰。 CD31在血管週細胞及內皮細胞均有錶達；VEGF 主要在內皮細胞錶達。結論改進的視網膜血管鋪片技術可成功應用于不同實驗動物，為視網膜血管性疾病的研究提供瞭重要途徑。
목적：탐토시망막혈관포편기술적개진급재실험동물소서，대서，구화후중적응용。방법응용단백매 K －이단백매연합소화법제비시망막혈관포편，행 HE 급 PAS 염색관찰혈관형태，면역조화방법관찰 CD31급VEGF 적표체。결과용개진방법제비적시망막혈관포편，혈관망완정，무신경조직잔류，가청석현시혈관주향。각급혈관형태청석，혈관분지청초완정，무단렬현상。혈관내피세포형태청석。 CD31재혈관주세포급내피세포균유표체；VEGF 주요재내피세포표체。결론개진적시망막혈관포편기술가성공응용우불동실험동물，위시망막혈관성질병적연구제공료중요도경。
Objective To improve the method of retinal vascular preparation and application in experimental animals of mice, rats, dogs and monkeys.Methods The retinal vessels were isolated with proteinase K-trypsin digestion technique.The samples were stained by hematoxylin-eosin(HE) and Periodic-Acid Schiff(PAS).The expression of CD31 and VEGF were detected by immunohistochemistry(IHC).Results The spread sheets of vascular network are complete, and can clearly show the blood vessels.No nerve tissue residue is left.Different levels of vascular morphology are clear. The vascular branches are clear and complete with no fracture.The cell morphology is intact, and the cell nucleus is clearly dispayed.The entire process can be completed in a short time, and has a high success rate.The vascular antigen are successfully retained: Expression of CD31 can be seen in both pericytes and endothelial cells; VEGF is mainly expressed in endothelial cells.Conclusions The improved method is successfully applied in different experimental animals.This approach will provide an important contribution to the retinal vascular disease research.
