CAJ | 학술논문

目的：探讨亲代三丁基锡暴露对 F1昆明小鼠血常规的影响。方法将雌、雄各40只小鼠随机分别分为空、低、中、高浓度组（0、0．2、2和20μg／kg），每天进行染毒，持续45 d。在第60天时，将同浓度组的雌：雄鼠按1：1进行同笼配种。仔鼠出生60 d 后取血，进行血常规的检测。结果与对照组相比，F1代雄性低浓度和高浓度组的红细胞数量和血红蛋白量显著增加（P ＜0．01）；F1代雄性低浓度组红细胞体积、平均血红蛋白含量（P ＜0．01）和淋巴细胞绝对值（P ＜0．05）显著降低；F1代雌性高浓度组的红细胞数量显著增加（P ＜0．01）， F1代雌性小鼠的血红蛋白量、红细胞压积、血小板量三项指标随着 TBT 浓度的增加呈剂量依赖性。结论亲代 TBT 暴露影响 F1代小鼠的血常规，且低浓度时对 F1代雄性小鼠的影响最大，高浓度时对 F1代雌性小鼠的影响最大。
목적：탐토친대삼정기석폭로대 F1곤명소서혈상규적영향。방법장자、웅각40지소서수궤분별분위공、저、중、고농도조（0、0．2、2화20μg／kg），매천진행염독，지속45 d。재제60천시，장동농도조적자：웅서안1：1진행동롱배충。자서출생60 d 후취혈，진행혈상규적검측。결과여대조조상비，F1대웅성저농도화고농도조적홍세포수량화혈홍단백량현저증가（P ＜0．01）；F1대웅성저농도조홍세포체적、평균혈홍단백함량（P ＜0．01）화림파세포절대치（P ＜0．05）현저강저；F1대자성고농도조적홍세포수량현저증가（P ＜0．01）， F1대자성소서적혈홍단백량、홍세포압적、혈소판량삼항지표수착 TBT 농도적증가정제량의뢰성。결론친대 TBT 폭로영향 F1대소서적혈상규，차저농도시대 F1대웅성소서적영향최대，고농도시대 F1대자성소서적영향최대。
Objective To explore effects of parents exposure to TBT on blood routine of F1 generation mice. Methods 80 mice including 40 males and 40 females, were randomly divided into control groups (CK) , low dose groups (LTBT), middle dose groups (MTBT) and high dose groups (HTBT).They were given dose of TBT (0,0.2,2, 20μg/kg) every day.The experiment lasted 45 days.At 60 days, one female and one male of the same concentration were bred in the same cage according to 1∶1.At postnatal day 60, blood was collected for the determination of blood routine. Results Compared with control group, the number of red blood cells and hemoglobin of F1 generation male mice in LTBT and HTBT groups were significantly increased (P <0.01); Red blood cell volume, mean corpuscular hemoglobin (P <0.01), and the lymphocyte absolute value in F1 generation male LTBT were significantly reduced (P <0.05); HTBT of female mice were significantly increased about the number of red blood cells (P <0.01).A dose-dependent increase of the hemoglobin, red blood cells, and platelet count of F1 generation female experimental groups was observed.Conclusion Parental TBT exposure affects the F1 mice blood routine.There is the greatest influence on LTBT in F1 generation male mice and on HTBT in F1 generation female mice.